Human/Mouse/Rat ERK4 Antibody

Catalog # Availability Size / Price Qty
MAB3914
MAB3914-SP
Detection of Human and Mouse ERK4 by Western Blot.
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Human/Mouse/Rat ERK4 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat ERK4.
Source
Monoclonal Rat IgG2A Clone # 355810
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ERK4
Met1-Ala126
Accession # P31152
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse ERK4 antibody by Western Blot. View Larger

Detection of Human and Mouse ERK4 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ERK4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry ERK4 antibody in Raji Human Cell Line by Immunocytochemistry (ICC). View Larger

ERK4 in Raji Human Cell Line. ERK4 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK4

Extracellular signal-regulated kinase 4 (ERK4) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 4 (MAPK4), p63MAPK, and ERK3‑related kinase. ERK4 and the structurally similar ERK3 (MAPK6) lack the phosphoacceptor sequence T-X-Y present in other MAPKs. ERK4 complexes with ERK3 to activate MAPK-activated protein kinase 5 (MK5), also known as PRAK.

Long Name
Extracellular Signal-regulated Kinase A
Entrez Gene IDs
5596 (Human); 225724 (Mouse); 54268 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.24; ERK3; Erk3-related; ERK4; ERK-4; Extracellular signal-regulated kinase 4; MAP kinase 4; MAP kinase isoform p63; MAPK 4; MAPK4; mitogen-activated protein kinase 4; p63Mapk; PRKM4; PRKM4p63-MAPK

Product Datasheets

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