Extracellular signal-regulated kinase 4 (ERK4) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 4 (MAPK4), p63MAPK, and ERK3‑related kinase. ERK4 and the structurally similar ERK3 (MAPK6) lack the phosphoacceptor sequence T-X-Y present in other MAPKs. ERK4 complexes with ERK3 to activate MAPK-activated protein kinase 5 (MK5), also known as PRAK.
Human/Mouse/Rat ERK4 Antibody
R&D Systems | Catalog # MAB3914
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 355810
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Product Specifications
Immunogen
E. coli-derived recombinant human ERK4
Met1-Ala126
Accession # P31152
Met1-Ala126
Accession # P31152
Specificity
Detects human, mouse, and rat ERK4.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat ERK4 Antibody
Detection of Human and Mouse ERK4 by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ERK4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
ERK4 in Raji Human Cell Line.
ERK4 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse/Rat ERK4 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and Raji human Burkitt's lymphoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and Raji human Burkitt's lymphoma cell line
Western Blot
2 µg/mL
Sample: LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line
Sample: LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ERK4
Long Name
Extracellular Signal-regulated Kinase A
Alternate Names
MAPK4, p63Mapk, PRKM4
Gene Symbol
MAPK4
UniProt
Additional ERK4 Products
Product Documents for Human/Mouse/Rat ERK4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat ERK4 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse/Rat ERK4 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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