Fibromodulin/FMOD Antibody
R&D Systems | Catalog # AF5945
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Asp75-Ile376
Accession # Q06828
Specificity
Clonality
Host
Isotype
Scientific Data Images for Fibromodulin/FMOD Antibody
Detection of Human/Mouse/Rat Fibromodulin by Western Blot.
Western blot shows lysates of human cartilage, human ovarian cancer, and rat uterine tissue. PVDF membrane was probed with 0.3 µg/mL of Sheep Anti-Human/Mouse/Rat Fibromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5945) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibromodulin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Detection of Zebrafish Fibromodulin/FMOD by Immunohistochemistry
SLRPs are enriched in human spinal cord lesions.Anti-LUM, anti-PRELP, anti-CHAD, and anti-FMOD immunoreactivity is increased (arrowheads; see calibration bar of lookup table (LUT)) in the epicenter of the injured human spinal cord at nine days-post injury, as compared to rostral control segments of the same patient. Note that immunoreactivity is mainly observed in proximity to the hemorrhage (black; see calibration bar of LUT). Also note that with the exception of blood-derived autofluorescence, individual channels show distinct pattern of immunofluorescence (IF) signal (insets). Shown are transversal sections (dorsal is up). Similar results were obtained for anti-LUM in five out of six cases, for anti-PRELP and anti-FMOD in four out of six cases, and anti-CHAD in two out of six cases (Supplementary Table 3). Scale bars: 2 mm, 200 µm (insets). The human icon in the panel was created using BioRender. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37884489), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Fibromodulin/FMOD Antibody
Western Blot
Sample: Human cartilage, human ovarian cancer, and rat uterine tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Fibromodulin/FMOD
Alternate Names
Gene Symbol
UniProt
Additional Fibromodulin/FMOD Products
Product Documents for Fibromodulin/FMOD Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Fibromodulin/FMOD Antibody
For research use only
Related Research Areas
Citations for Fibromodulin/FMOD Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars