Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody
Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody Summary
Ser542-Thr874
Accession # AAH57870
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
HIF‑2 alpha /EPAS1 in Human Prostate Cancer Tissue. HIF-2a/EPAS1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
View Larger
HIF‑2 alpha /EPAS1 in Mouse Testis. HIF-2a/EPAS1 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Human/Mouse/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
View Larger
Detection of Human, Mouse, and Rat HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 hours. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2a/EPAS1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-2 alpha/EPAS1
The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.
Product Datasheets
Citations for Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
19
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Hypoxia promotes an inflammatory phenotype of fibroblasts in pancreatic cancer
Authors: AM Mello, T Ngodup, Y Lee, KL Donahue, J Li, A Rao, ES Carpenter, HC Crawford, M Pasca di M, KE Lee
Oncogenesis, 2022;11(1):56.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The HIFalpha-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells
Authors: A Jatho, A Zieseniss, K Brechtel-C, J Guo, KO Böker, G Salinas, RH Wenger, DM Katschinsk
Cells, 2022;11(4):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The renal cancer risk allele at 14q24.2 activates a novel hypoxia-inducible transcription factor-binding enhancer of DPF3 expression
Authors: J Protze, S Naas, R Krüger, C Stöhr, A Kraus, S Grampp, M Wiesener, M Schiffer, A Hartmann, B Wullich, J Schödel
The Journal of Biological Chemistry, 2022;0(0):101699.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting HIF-activated collagen prolyl 4-hydroxylase expression disrupts collagen deposition and blocks primary and metastatic uveal melanoma growth
Authors: S Kaluz, Q Zhang, Y Kuranaga, H Yang, S Osuka, D Bhattachar, NS Devi, J Mun, W Wang, R Zhang, MM Goodman, HE Grossnikla, EG Van Meir
Oncogene, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity
Authors: W Yang, T Yu, X Huang, AJ Bilotta, L Xu, Y Lu, J Sun, F Pan, J Zhou, W Zhang, S Yao, CL Maynard, N Singh, SM Dann, Z Liu, Y Cong
Nat Commun, 2020;11(1):4457.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
TET-Mediated Hypermethylation Primes SDH-Deficient Cells for HIF2&alpha-Driven Mesenchymal Transition
Authors: A Morin, J Goncalves, S Moog, LJ Castro-Veg, S Job, A Buffet, MJ Fontenille, J Woszczyk, AP Gimenez-Ro, E Letouzé, J Favier
Cell Rep, 2020;30(13):4551-4566.e7.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions
Authors: K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama
J Neuroinflammation, 2020;17(1):141.
Species: Rat
Sample Types: Cell Lysate
Applications: Western Blot -
The lncRNA Neat1 promotes activation of inflammasomes in macrophages
Authors: P Zhang, L Cao, R Zhou, X Yang, M Wu
Nat Commun, 2019;10(1):1495.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
PLoS ONE, 2018;13(12):e0208579.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin
Authors: H Kobayashi, Q Liu, TC Binns, AA Urrutia, O Davidoff, PP Kapitsinou, AS Pfaff, H Olauson, A Wernerson, AB Fogo, GH Fong, KW Gross, VH Haase
J Clin Invest, 2016;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Unprocessed Interleukin-36alpha Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1.
Authors: Milora K, Fu H, Dubaz O, Jensen L
J Invest Dermatol, 2015;135(12):2992-3000.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
Authors: Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard-O'Riley R, Wilson T, Rankin E, Giaccia A, Schipani E
Dev Biol, 2014;393(1):124-36.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
IL-4 reduces the proangiogenic capacity of macrophages by down-regulating HIF-1alpha translation.
Authors: Dehne N, Tausendschon M, Essler S, Geis T, Schmid T, Brune B
J Leukoc Biol, 2014;95(1):129-37.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
J Leukoc Biol, 2014;96(3):365-75.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-beta1 processing.
Authors: Wottawa M, Leisering P, Ahlen M, Schnelle M, Vogel S, Malz C, Bordoli M, Camenisch G, Hesse A, Napp J, Alves F, Kristiansen G, Farhat K, Katschinski D
Int J Cancer, 2014;132(12):2787-98.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
HIF-1alpha activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells.
Authors: Weidemann A, Breyer J, Rehm M, Eckardt K, Daniel C, Cicha I, Giehl K, Goppelt-Struebe M
Cell Commun Signal, 2013;11(0):80.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
BMC Cancer, 2012;12(0):201.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hypoxia-inducible Factor-1 (HIF-1) but Not HIF-2 Is Essential for Hypoxic Induction of Collagen Prolyl 4-Hydroxylases in Primary Newborn Mouse Epiphyseal Growth Plate Chondrocytes.
Authors: Aro E, Khatri R
J. Biol. Chem., 2012;287(44):37134-44.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Loss of myeloid cell-derived vascular endothelial growth factor accelerates fibrosis.
Authors: Stockmann C, Kerdiles Y, Nomaksteinsky M, Weidemann A, Takeda N, Doedens A, Torres-Collado AX, Iruela-Arispe L, Nizet V, Johnson RS
Proc. Natl. Acad. Sci. U.S.A., 2010;107(9):4329-34.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody and earn rewards!
Have you used Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
