Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody

(10 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat HIF-2 alpha /EPAS1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse HIF-2 alpha /EPAS1
    Ser542-Thr874
    Accession # AAH57870
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

HIF‑2 alpha /EPAS1 in Human Prostate Cancer Tissue. HIF‑2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

HIF‑2 alpha /EPAS1 in Mouse Testis. HIF‑2 alpha /EPAS1 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human, Mouse, and Rat
HIF‑2 alpha /EPAS1 by Western Blot.
Western blot shows lysates of
MCF‑7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2 for 16 hours. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse HIF‑2 alpha /EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF‑2 alpha /EPAS1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-2 alpha/EPAS1
The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.
  • Long Name:
    Hypoxia-inducible Transcription Factor 2 alpha
  • Entrez Gene IDs:
    2034 (Human)
  • Alternate Names:
    Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; bHLHe73ECYT4; Class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF2AHIF-2-alpha; HLF; HLFHIF-1-alpha-like factor; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; MOP2HIF2-alpha; PASD2PAS domain-containing protein 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin
    Authors: H Kobayashi, Q Liu, TC Binns, AA Urrutia, O Davidoff, PP Kapitsinou, AS Pfaff, H Olauson, A Wernerson, AB Fogo, GH Fong, KW Gross, VH Haase
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Unprocessed Interleukin-36alpha Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1.
    Authors: Milora K, Fu H, Dubaz O, Jensen L
    J Invest Dermatol, 2015;135(12):2992-3000.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  3. IL-4 reduces the proangiogenic capacity of macrophages by down-regulating HIF-1alpha translation.
    Authors: Dehne N, Tausendschon M, Essler S, Geis T, Schmid T, Brune B
    J Leukoc Biol, 2014;95(1):129-37.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
    Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
    J Leukoc Biol, 2014;96(3):365-75.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
    Authors: Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard-O'Riley R, Wilson T, Rankin E, Giaccia A, Schipani E
    Dev Biol, 2014;393(1):124-36.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-beta1 processing.
    Authors: Wottawa M, Leisering P, Ahlen M, Schnelle M, Vogel S, Malz C, Bordoli M, Camenisch G, Hesse A, Napp J, Alves F, Kristiansen G, Farhat K, Katschinski D
    Int J Cancer, 2014;132(12):2787-98.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. HIF-1alpha activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells.
    Authors: Weidemann A, Breyer J, Rehm M, Eckardt K, Daniel C, Cicha I, Giehl K, Goppelt-Struebe M
    Cell Commun Signal, 2013;11(0):80.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Hypoxia-inducible Factor-1 (HIF-1) but Not HIF-2 Is Essential for Hypoxic Induction of Collagen Prolyl 4-Hydroxylases in Primary Newborn Mouse Epiphyseal Growth Plate Chondrocytes.
    Authors: Aro E, Khatri R
    J. Biol. Chem., 2012;287(44):37134-44.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
    Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
    BMC Cancer, 2012;12(0):201.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Loss of myeloid cell-derived vascular endothelial growth factor accelerates fibrosis.
    Authors: Stockmann C, Kerdiles Y, Nomaksteinsky M, Weidemann A, Takeda N, Doedens A, Torres-Collado AX, Iruela-Arispe L, Nizet V, Johnson RS
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(9):4329-34.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
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