Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HIF-2 alpha/EPAS1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse HIF-2 alpha /EPAS1
Ser542-Thr874
Accession # AAH57870

Specificity

Detects human, mouse, and rat HIF-2 alpha /EPAS1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antibody

HIF-2a/EPAS1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

HIF‑2 alpha /EPAS1 in Human Prostate Cancer Tissue.

HIF-2a/EPAS1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

HIF-2a/EPAS1 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr).

HIF‑2 alpha /EPAS1 in Mouse Testis.

HIF-2a/EPAS1 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Human/Mouse/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human, Mouse, and Rat HIF-2a/EPAS1 antibody by Western Blot.

Detection of Human, Mouse, and Rat HIF‑2 alpha /EPAS1 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 hours. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2a/EPAS1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse HIF-2 alpha/EPAS1 by Western Blot

Detection of Mouse HIF-2 alpha/EPAS1 by Western Blot

Kidney-derived Sca-1+ cells produce Epo. (A) Epo protein levels in supernatants obtained from Sca-1+ cells cultured in normoxia or hypoxia (n = 3 biological replicates). (B) HIF-1 alpha and HIF-2 alpha protein levels in Sca-1+ kidney-derived cells after exposure to normoxia (20% O2) or hypoxia (1% O2). Numbers below the blots indicate the fold change of the ratio of HIF-1 alpha /Tubulin and HIF-2 alpha /Tubulin in 1% O2 to the respective 20% O2 control. (C) Epo RNA levels in Sca-1+ cells that were incubated either at 20% O2 of 1% O2 as indicated (n = 4 biological replicates). (D) Mesenchymal stem cell markers in renal Sca-1+ cells on day 21 of culture; reads per kilobase million (RPKM). (E) Volcano plot of 4592 significantly up- and 5376 significantly downregulated mRNAs in kidney-derived Sca-1+ cells on day 21 in culture compared to day 0. Mean values ± SEM are shown. * p < 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35203399), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of HIF-2 alpha /EPAS1 by Western Blot

Detection of HIF-2 alpha /EPAS1 by Western Blot

Hypoxia upregulates AK4 in an HIF-1 alpha -dependent manner. (A) Representative Western blot for HIF-1 alpha and AK4 followed by densitometric quantification of relative expression in PASMCs treated with 50 µM DFO for 24 h, compared to untreated control; n = 4. (B) Representative Western blot for HIF-1 alpha and AK4 and densitometric quantification of relative expression in PASMCs transfected with HIF-1 alpha siRNA (siHIF-1 alpha ), followed by hypoxic (HOX, 1% O2) exposure for 24 h, compared to non-targeting siRNA as a control (scrambled siRNA = scramble) under NOX or HOX; n = 3. (C,E) Relative mRNA expression of AK4 in PASMCs transfected with siHIF-1 alpha (C) or siEPAS1 (E), followed by hypoxic (HOX, 1% O2) exposure for 24 h, compared to control (scramble); n = 3–5. (D) Representative Western blot for EPAS1 (HIF-2 alpha ) and AK4 and densitometric quantification of relative expression in PASMCs transfected with EPAS1 siRNA (siEPAS1), followed by hypoxic (HOX, 1% O2) exposure for 24 h, compared to control (scramble) under NOX or HOX; n = 4. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p< 0.0001, (A,C,E) unpaired Student’s t-test or (B,D) one-way ANOVA followed by Tukey multiple comparisons test. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/22/19/10371), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13-15 d.p.c.), immersion fixed paraffin-embedded sections of human prostate cancer tissue, and perfusion fixed frozen sections of mouse testis

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 3 using AF2997 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIF-2 alpha/EPAS1

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.

Long Name

Hypoxia-inducible Transcription Factor 2 alpha

Alternate Names

EPAS1, HIF 2A, HIF2 alpha, HIF2A, HLF, MOP2

Entrez Gene IDs

2034 (Human); 13819 (Mouse)

Gene Symbol

EPAS1

UniProt

AAH57870

Additional HIF-2 alpha/EPAS1 Products

Product Documents for Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antibody

For research use only

Citations for Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antibody

Customer Reviews for Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antibody (1)

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  • hif2alpha expression in pasmc
    Name: Anonymous
    Application: Western Blot
    Sample Tested: pulmonary arterial smooth muscle cells
    Species: Human
    Verified Customer | Posted 09/08/2025
    Hif2a in normoxic and hypoxic conditions of treated h pasmc
    used the hpasmc to check the expression of hif1a in normoxia and hypoxia
    Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antibody AF2997

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