Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody

Catalog # Availability Size / Price Qty
AF7679
AF7679-SP
Detection of Human, Mouse, and Rat Histone Deacetylase 2/HDAC2 by Western Blot.
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Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Histone Deacetylase 2/HDAC2 in Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human HDAC1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Histone Deacetylase 2/HDAC2
Pro386-Pro488
Accession # Q92769
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat Histone Deacetylase 2/HDAC2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat Histone Deacetylase 2/HDAC2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Rat‑2 rat embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7679) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histone Deacetylase 2/HDAC2

HDAC2 (Histone deacetylase 2; also HD2) is a 55-60 kDa nuclear member of the class I subfamily, histone deacetylase family of transcriptional regulators. It is found in a variety of cell types including monocytes, cardiac muscle cells and neurons. HDAC2 is also crucial for embryonic development. HDAC2 catalyzes the hydrolytic release of acetyl groups from acetylated histone proteins. Within the context of a large complex, this has the effect of repressing gene activity. Human HDAC2 is 488 amino acids (aa) in length. It contains one histone deacetylation domain (aa 29-388), plus sites for phosphorylation, nitrosylation, ubiquitination, and nitration. There is one alternative start site at Met31. Over aa 386-488, human HDAC2 shares 98% and 53% aa sequence identity with mouse HDAC2 and human HDAC1, respectively.

Long Name
Histone Deacetylase 2
Entrez Gene IDs
3066 (Human); 15182 (Mouse); 84577 (Rat)
Alternate Names
EC 3.5.1.98; HD2; HDAC2; Histone Deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1

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