Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Histone Deacetylase 2/HDAC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Histone Deacetylase 2/HDAC2
Pro386-Pro488
Accession # Q92769

Specificity

Detects human, mouse, and rat Histone Deacetylase 2/HDAC2 in Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human HDAC1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody

Detection of Human, Mouse, and Rat Histone Deacetylase 2/HDAC2 antibody by Western Blot.

Detection of Human, Mouse, and Rat Histone Deacetylase 2/HDAC2 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Rat-2 rat embryonic fibroblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7679) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Rat‑2 rat embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Histone Deacetylase 2/HDAC2

HDAC2 (Histone deacetylase 2; also HD2) is a 55-60 kDa nuclear member of the class I subfamily, histone deacetylase family of transcriptional regulators. It is found in a variety of cell types including monocytes, cardiac muscle cells and neurons. HDAC2 is also crucial for embryonic development. HDAC2 catalyzes the hydrolytic release of acetyl groups from acetylated histone proteins. Within the context of a large complex, this has the effect of repressing gene activity. Human HDAC2 is 488 amino acids (aa) in length. It contains one histone deacetylation domain (aa 29-388), plus sites for phosphorylation, nitrosylation, ubiquitination, and nitration. There is one alternative start site at Met31. Over aa 386-488, human HDAC2 shares 98% and 53% aa sequence identity with mouse HDAC2 and human HDAC1, respectively.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Entrez Gene IDs

3066 (Human); 15182 (Mouse); 84577 (Rat)

Gene Symbol

HDAC2

UniProt

Q92769 (Human)

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody

For research use only

Customer Reviews for Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody and earn rewards!

Have you used Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail