Human/Mouse/Rat MEK1 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB2079N Datasheet / COA / SDS

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FAB2079N-100UG

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Human/Mouse/Rat MEK1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MEK1 in Western blots. In Western blots, no crossreactivity with recombinant human MEK2 is observed.

Source

Monoclonal Mouse IgG2a Clone # 239421

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human MEK1
Lys2-Val393 (Ser218Asp, Ser222Asp)
Accession # Q02750

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MEK1

Mitogen-Activated Protein Kinase Kinase 1 (MEK1 or MAP2K1), also known as MKK1, belongs to the family of STE kinases. Both MEK1 and the related MEK2 aredual‑specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK1 by Raf occurs through phosphorylation at S218 and S222.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Entrez Gene IDs

5604 (Human); 26395 (Mouse)

Alternate Names

dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)