Detection of Human/Mouse/Rat MEK1 by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat MEK1 Monoclonal Antibody (Catalog # MAB2079) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mitogen-Activated Protein Kinase Kinase 1 (MEK1 or MAP2K1), also known as MKK1, belongs to the family of STE kinases. Both MEK1 and the related MEK2 aredual‑specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK1 by Raf occurs through phosphorylation at S218 and S222.
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