Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MIF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse MIF
Pro2-Ala115
Accession # NP_034928

Specificity

Detects human and mouse MIF in direct ELISAs and human, mouse and rat MIF in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat MIF Antibody

Detection of Human, Mouse, and Rat MIF antibody by Western Blot.

Detection of Human, Mouse, and Rat MIF by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1978) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat MIF Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: U937 human histiocytic lymphoma cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and NR8383 rat alveolar macrophage cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIF

MIF (Macrophage Inhibitory Factor; also GIF) is a 12-13 kDa member of the MIF family of proteins. Although secreted, it possesses no definitive signal sequence. MIF is widely expressed, and serves to regulate immune reactions. On macrophages and B cells, MIF binds to a CD74:CD44 complex, initiating downstream signaling. On monocytes, this promotes TNF-alpha production plus IFN-gamma -stimulated NO production. On B cells, this promotes B cell survival. MIF also binds to leukocyte CD74:CXCR2 and CD74:CXCR4 heterodimers, initiating T cell and monocyte migration. A tautomerase activity has also been shown for MIF, but the significance of this is unclear. Mouse MIF is 114 amino acids (aa) in length. Its tautomerase activity is suggested to involve the entire length of the molecule. MIF is phosphorylated at Ser91, and an additional, singular Cys residue is covalently linked to MIF at Cys60. MIF is considered to act as a homotrimer. Full-length mouse MIF shows 90% and 99% aa sequence identity with human and rat MIF, respectively.

Long Name

Macrophage Migration Inhibitory Factor

Alternate Names

EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase

Entrez Gene IDs

4282 (Human); 17319 (Mouse)

Gene Symbol

MIF

UniProt

NP_034928

Additional MIF Products

Product Documents for Human/Mouse/Rat MIF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat MIF Antibody

For research use only

Citations for Human/Mouse/Rat MIF Antibody

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