Human/Mouse/Rat NCOR1 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat NCOR1 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human NCOR1
    Gln1770-Ala1947
    Accession # O75376
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat NCOR1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat NCOR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6167) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NCOR1 at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NCOR1

NCOR1 (Nuclear receptor Co-Repressor 1) is a 270 kDa member of the NCoR family of molecules. It is widely expressed, being found in hepatocytes, intestinal crypt cells, neural stem cells, plus immature thymocytes and erythrocytes. NCOR1 is a transcriptional repressor. It forms a complex with HDAC3, TAB2 and ZBTB33, and interacts with a ligand‑independent THR:RXR heterodimer bound to select gene promoters. Human NCOR1 is 2440 amino acids (aa) in length. It possesses one N‑terminal repression domain (aa 1‑312), two DNA‑binding SANT domains (aa 437‑674) and a second repression domain (aa 737‑1004). Multiple Ser, Thr and Tyr phosphorylation sites exist that regulate complex dissociation. There are multiple potential splice variants. Short poly Lys motifs serve as substitutions for the C‑terminal 1900‑1910 amino acids. There is also a 16 aa insertion after Glu727, coupled to either a Ile substitution for aa 1842‑1961, or a six aa substitution for aa 31‑145. Over aa 1770‑1947, human NCOR1 shares 96% aa identity with mouse NCOR1.

  • Long Name:
    Nuclear Receptor Co-repressor 1
  • Entrez Gene IDs:
    9611 (Human); 20185 (Mouse)
  • Alternate Names:
    hN-CoR; KIAA1047; KIAA1047N-CoR1; MGC104216; N-CoR; NCOR1; N-CoRhCIT529I10; nuclear receptor corepressor 1; nuclear receptor co-repressor 1; thyroid hormone- and retinoic acid receptor-associated corepressor 1; TRAC1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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