Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all NDFIP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NDFIP1
Ala10-Asp105
Accession # Q9BT67

Specificity

Detects human, mouse and rat NDFIP1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat NDFIP1 Antibody

Detection of Human, Mouse, and Rat NDFIP1 antibody by Western Blot.

Detection of Human, Mouse, and Rat NDFIP1 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, SK-Mel-28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1-6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NDFIP1 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NDFIP1 antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

NDFIP1 in SH‑SY5Y Human Cell Line.

NDFIP1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat NDFIP1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line

Western Blot

2 µg/mL
Sample: A172 human glioblastoma cell line, SK‑Mel‑28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1‑6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NDFIP1

NDFIP1 (NEDD4 family-interacting protein 1 and NEDD4 WW domain-binding protein 5) is a 26 kDa widely expressed protein. NDFIP1 is thought to be part of a family of integral Golgi membrane proteins. It serves as an adaptor for several E3 ubiquitin ligases including NEDD4 and ITCH, and consequently modulates the stability of their targets. NDFIP1 is genetically linked to human allergic and autoimmune disease, but the cellular mechanism by which these proteins enable foreign and self-antigens to be tolerated is unresolved. Over aa10-105, human NDFIP1 is 95% identical to mouse and rat NDFIP1.

Long Name

Nedd4 Family Interacting Protein 1

Alternate Names

N4WBP5

Entrez Gene IDs

80762 (Human); 65113 (Mouse); 291609 (Rat)

Gene Symbol

NDFIP1

UniProt

Q9BT67

Additional NDFIP1 Products

Product Documents for Human/Mouse/Rat NDFIP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat NDFIP1 Antibody

For research use only

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Protocols

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