Human/Mouse/Rat Neuropilin-2 MAb (Clone 96009)

Name: Human/Mouse/Rat Neuropilin-2 MAb (Clone 96009)
Brand: R&D Systems
SKU: MAB567

Catalog #: MAB567 Datasheet / COA / SDS

Discontinued Product

MAB567 has been discontinued.

View all Neuropilin-2 products.

Detection of Human, Mouse, and Rat Neuropilin‑2 by Western Blot.

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Human/Mouse/Rat Neuropilin-2 MAb (Clone 96009) Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects rat Neuropilin-2 in direct ELISAs and detects human, mouse, and rat Neuropilin-2 in Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Neuropilin‑1.

Source

Monoclonal Mouse IgG_2B Clone # 96009

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Neuropilin‑2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, C6 rat glioma cell line, bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Neuropilin-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of Neuropilin-2 in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Simple Western

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Detection of Rat Neuropilin‑2 by Simple Western^TM. Simple Western lane view shows lysates of C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin‑2 at approximately 139 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Neuropilin‑2 Monoclonal Antibody (Catalog # MAB567). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-2

Neuropilin-1 (Npn-1, previously known as neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely non-overlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF₁₆₅. Npn-1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

References

Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
Neufeld, G. et al. (1999) FASEB J. 13:9.
Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.

Entrez Gene IDs

8828 (Human); 18187 (Mouse); 81527 (Rat)

Alternate Names

MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.