Human/Mouse/Rat NM23-H1/H2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat NM23-H1/H2 by Western Blot.
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Product Details
Supplemental Products

Human/Mouse/Rat NM23-H1/H2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat NM23‑H1/H2 in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human NM23‑H1
Accession # P15531
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat NM23-H1/H2 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat NM23-H1/H2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.2 µg/mL of Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23-H1/H2 at approximately 17-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry NM23-H1/H2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

NM23‑H1/H2 in MCF‑7 Human Cell Line. NM23-H1/H2 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human/Mouse/Rat NM23-H1/H2 Polyclonal Antibody (Catalog # AF6019) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NM23-H1/H2

NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 17‑20 kDa member of the NDK family of enzymes. NM23‑H1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23‑H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows histidine protein and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2  (Non-metastatic protein 23 homolog 2; also NDKB), and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N-terminus has also been described.

Long Name
Non-metastatic Protein 23 Homolog 1/Homolog 2
Entrez Gene IDs
4830 (Human)
Alternate Names
AL024257;AWD;GAAD;NB;NBS;NDKA;NDKB;NDPKA;NDPK-A;NDPKB;NDPK-B;NM23;nm23-2;NM23A;NM23B;NM23-H1;NM23-H2;NM23-M1;nm23-M2;NME1;NME2;p18-12d;PUF; NM23-H1/H2; NM23H2

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