Human/Mouse/Rat PCK1 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, rat PCK1 in Western blots, and detects recombinant human PCK1 in direct ELISAs. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse PCK1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PCK1
    Met1-Ile88
    Accession # P35558
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat PCK1 by Western Blot. Western blot shows lysates of human liver tissue, human kidney tissue, mouse kidney tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7316) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PCK1

PCK1 (Phosphoenolpyruvate carboxykinase, cytosolic; also PEPCK-C) is a 67-69 kDa cytosolic member of the phosphoenolpyruvate kinase (GTP) family of enzymes. It is found in hepatocytes, adipocytes, intestinal and renal proximal tubule epithelial cells. PCK1 is one of two enzymes that drive the conversion of oxaloacetate plus GTP into PEP, GDP and CO2. The PEP generated in this reaction forms glucose via fructose phosphate intermediates. The other enzyme that has this activity is termed PEPCK-M and found in mitochondria. Human PCK1 is 622 amino acids (aa) in length. It contains a large catalytic region (aa 27-615) and three utilized acetylation sites. There is one potential alternative start site at Met315. Over aa 1-88, human PCK1 shares 80% aa sequence identity with mouse PCK1.

  • Long Name:
    Phosphoenolpyruvate Carboxykinase 1
  • Entrez Gene IDs:
    5105 (Human); 18534 (Mouse); 362282 (Rat)
  • Alternate Names:
    EC 4.1.1.32; MGC22652; PCK1; PEPCK1; PEPCKC; PEPCK-C; PEPCK-CPEP carboxykinase; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic; Phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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