Human/Mouse/Rat PDHX Antibody
Human/Mouse/Rat PDHX Antibody Summary
Accession # P15374
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat PDHX by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, mouse heart tissue, and rat heart tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PDHX Polyclonal Antibody (Catalog # AF6014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PDHX at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH-L3 cleaves both monomeric ubiquitin (Ub) from Ub-protein conjugates, and a GGLRQ peptide from the C-terminus of the Ub-like protein NEDD-8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179 - 230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH-L3.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse/Rat PDHX Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat PDHX Antibody and earn rewards!
Have you used Human/Mouse/Rat PDHX Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image