Human/Mouse/Rat PDHX Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat PDHX by Western Blot.
1 Image
Product Details
Supplemental Products

Human/Mouse/Rat PDHX Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat PDHX in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PDHX
aa 387-501
Accession # P15374
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat PDHX antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat PDHX by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, mouse heart tissue, and rat heart tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PDHX Polyclonal Antibody (Catalog # AF6014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PDHX at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDHX

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH-L3 cleaves both monomeric ubiquitin (Ub) from Ub-protein conjugates, and a GGLRQ peptide from the C-terminus of the Ub-like protein NEDD-8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179 - 230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH-L3.

Long Name
Pyruvate Dehydrogenase Complex, Component X
Entrez Gene IDs
8050 (Human); 27402 (Mouse); 311254 (Rat)
Alternate Names
Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex; DLDBP; E3-binding protein; E3BP; E3BP1; E3BPpyruvate dehydrogenase complex, E3-binding protein subunit; OPDX; PDHX; PDX1; PDX1Lipoyl-containing pyruvate dehydrogenase complex component X; proX; proXpyruvate dehydrogenase complex, lipoyl-containing component X; pyruvate dehydrogenase complex, component X; pyruvate dehydrogenase protein X component, mitochondrial

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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