Human/Mouse/Rat Phospho-MKK4 (S257/T261) Alexa Fluor® 532-conjugated Antibody

Catalog #: AF2990X Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2990X-100UG

Save 15% on Select RUO Reagents. See Details.

All MKK4 Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-MKK4 (S257/T261) Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MKK4 when dually phosphorylated at sites corresponding to S257 and T261 of human MKK4.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human MKK4 S257/T261 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MKK4

Mitogen-activated protein kinase kinase 4 (MKK4 or MAP2K4), also known as SAPK/ERK kinase 1 (SEK1) and Jun kinase kinase 1 (JNKK1), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser257 and Thr261 by several upstream MAPK kinase kinases (MAP3Ks). MKK4 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.

Long Name

Mitogen-activated Protein Kinase Kinase 4

Entrez Gene IDs

6416 (Human)

Alternate Names

c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; JNKK1MAPK/ERK kinase 4; MAP kinase kinase 4; MAP2K4; MAPKK4; MEK4; MEK4MEK 4; mitogen-activated protein kinase kinase 4; MKK4; MKK4SAPK/ERK kinase 1; PRKMK4; PRKMK4MAPKK 4; SEK1; SERK1; SERK1EC 2.7.12.2