Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antibody

Catalog # Availability Size / Price Qty
Detection of Rat and Human Phospho-MKK4 (S257/T261) by Western Blot.
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Product Details
Supplemental Products

Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat MKK4 when dually phosphorylated at sites corresponding to S257 and T261 of human MKK4.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing human MKK4 S257/T261 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Rat and Human Phospho-MKK4 (S257/T261) by Western Blot. View Larger

Detection of Rat and Human Phospho-MKK4 (S257/T261) by Western Blot. Western blot shows lysates of PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 300 mM sorbitol for 30 minutes and HepG2 human hepatocellular carcinoma cell line untreated or treated with 10 ng/mL Recombinant Human IL-1 beta (Catalog # 201-LB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2990), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MKK4 (S257/T261) at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK4

MKK4, also known as MEK4, JNKK1, SEK1, MAP2K4, is an intracellular protein kinase that functions in the JNK (Jun N-terminal kinase) signal transduction pathway. In response to activation by one of several MAPKKKs, MKK4 (and MKK7) phosphorylates and activates the JNKs that regulate neural development, apoptosis, inflammation, and cellular responses to stress.

Long Name
Mitogen-activated Protein Kinase Kinase 4
Entrez Gene IDs
6416 (Human)
Alternate Names
c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; JNKK1MAPK/ERK kinase 4; MAP kinase kinase 4; MAP2K4; MAPKK4; MEK4; MEK4MEK 4; mitogen-activated protein kinase kinase 4; MKK4; MKK4SAPK/ERK kinase 1; PRKMK4; PRKMK4MAPKK 4; SEK1; SERK1; SERK1EC

Product Datasheets


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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control


Reconstitution Buffers

Secondary Antibodies

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