Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 479819
View all PLC-gamma 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLC-gamma 1
Phe550-His663
Accession # P19174

Specificity

Detects endogenous human, mouse, and rat PLC-gamma 1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat PLC‑ gamma 1 Antibody

Detection of Human/Mouse/Rat PLC-?1 antibody by Western Blot.

Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC- gamma1 Monoclonal Antibody (Catalog # MAB32881) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PLC- gamma1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PLC‑ gamma 1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue

Reviewed Applications

Read 1 review rated 5 using MAB32881 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PLC-gamma 1

PLC-gamma 1 (phospholipase C-gamma 1) cleaves the polar head group from the membrane lipid phosphatidyl-inositol 4,5-biphosphate. The consequence of this reaction is the generation of two secondary messengers, inositol 1,4,5-trisphosphate and diacylglycerol, which mediate the mobilization of intracellular calcium and the activation of protein kinase C, respectively. Members of the PLC family are regulated by different signaling pathways. PLC-gamma 1 is regulated through receptor and non-receptor tyrosine kinases, while the PLC-beta isoforms are regulated by G-protein coupled receptors.

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Entrez Gene IDs

5335 (Human); 18803 (Mouse); 25738 (Rat)

Gene Symbol

PLCG1

UniProt

P19174

Additional PLC-gamma 1 Products

Product Documents for Human/Mouse/Rat PLC‑ gamma 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat PLC‑ gamma 1 Antibody

For research use only

Customer Reviews for Human/Mouse/Rat PLC‑ gamma 1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse/Rat PLC‑ gamma 1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Mouse/Rat PLC-gamma 1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 01/20/2022
    Human/Mouse/Rat PLC‑ gamma 1 Antibody MAB32881

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies