Detection of Human, Mouse, and Rat PTK7/CCK4 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 171 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCK4 (colon carcinoma kinase 4, also known as PTK7) is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, CCK4 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner.
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