Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RNF2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RNF2
aa 147-228
Accession # Q99496

Specificity

Detects human, mouse, and rat RNF2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat RNF2 Antibody

Detection of Human, Mouse, and Rat RNF2 antibody by Western Blot.

Detection of Human, Mouse, and Rat RNF2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RNF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RNF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat RNF2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RNF2

RNF2 (RING [really interesting gene] finger protein 2/1B; also BAP-1, HIP-2 interacting protein 3 and DinG) is a 37 kDa member of the ring finger domain family of proteins. It is expressed in embryonic tissue, and serves as an E3 ubiquitin ligase within a polycomb complex that acts on histone H2A. In effect, RNF2 contributes to gene repression by blocking RNA pol II advance along gene promoters. RNF2 forms homodimers, and interacts with over a dozen proteins. Human RNF2 is 336 amino acids (aa) in length contains one Zn‑finger domain (aa 51‑91) and two phosphorylation sites at Ser168 and Ser203. There is one potential isoform that shows a deletion of aa 84‑155. Over aa 147‑228, human RNF2 shares 99% aa identity with mouse RNF2.

Long Name

Ring Finger Protein 2

Alternate Names

DING, HIPI3, RING1B, RING2

Entrez Gene IDs

6045 (Human); 19821 (Mouse); 304850 (Rat)

Gene Symbol

RNF2

UniProt

Q99496 (Human)

Additional RNF2 Products

Product Documents for Human/Mouse/Rat RNF2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat RNF2 Antibody

For research use only

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