Human/Mouse/Rat SHC1 Alexa Fluor™ Plus 680‑conjugated Antibody

Name: Human/Mouse/Rat SHC1 Alexa Fluor™ Plus 680‑conjugated Antibody
Brand: R&D Systems
SKU: FAB7129AFP680
Price: 559 USD
Availability: InStock

R&D Systems | Catalog # FAB7129AFP680

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 725001

View all SHC1 Primary Antibodies »

Product Specifications

Specificity

Detects human SHC1 in direct ELISAs and human, mouse, and rat SHC1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human SHC3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human/Mouse/Rat SHC1 Alexa Fluor™ Plus 680‑conjugated Antibody

Application

Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SHC1

SH2 domain-containing transforming protein C1 (SHC1), also known as ShcA, is a cytoplasmic adaptor protein that is important in the signal transduction from growth factor, cytokine, and lymphocyte antigen receptors. SHC1 contains a PTB/PID domain (aa 156-339), a collagen homology domain (aa 340-487), and an SH2 domain (aa 488-579). Alternate splicing generates additional isoforms that differ in the extent of N-terminal truncation. Activation of SHC1 by phosphorylation at Ser239, Ser240, and Tyr317 enables Shc1 to interact with the GRB2/SOS complex, leading to the transcription of genes involved in mitogenesis and apoptosis. The p46 and p52 isoforms promote mitogenic signaling, while the p66 isoform promotes apoptosis and functions as a negative regulator of SHC1-mediated mitogenic signal transduction. Within aa 488-579, human SHC1 shares 100% aa sequence identity with mouse and rat SHC1.

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Entrez Gene IDs

6464 (Human); 20416 (Mouse); 85385 (Rat)

Gene Symbol

SHC1

UniProt

P29353

Additional SHC1 Products

Product Documents for Human/Mouse/Rat SHC1 Alexa Fluor™ Plus 680‑conjugated Antibody

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Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat SHC1 Alexa Fluor™ Plus 680‑conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only