Human/Mouse/Rat SHC1 Antibody

Name: Human/Mouse/Rat SHC1 Antibody
Brand: R&D Systems
SKU: MAB7129
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB7129

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 725001

View all SHC1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SHC1
Pro379-Val470, Trp488-Val579
Accession # P29353

Specificity

Detects human SHC1 in direct ELISAs and human, mouse, and rat SHC1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human SHC3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse/Rat SHC1 Antibody

Detection of Human, Mouse, and Rat SHC1 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat SHC1 Monoclonal Antibody (Catalog # MAB7129) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for SHC1 at approximately 66, 52, and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat SHC1 Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: A172 human glioblastoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: SHC1

SH2 domain-containing transforming protein C1 (SHC1), also known as ShcA, is a cytoplasmic adaptor protein that is important in the signal transduction from growth factor, cytokine, and lymphocyte antigen receptors. SHC1 contains a PTB/PID domain (aa 156-339), a collagen homology domain (aa 340-487), and an SH2 domain (aa 488-579). Alternate splicing generates additional isoforms that differ in the extent of N-terminal truncation. Activation of SHC1 by phosphorylation at Ser239, Ser240, and Tyr317 enables Shc1 to interact with the GRB2/SOS complex, leading to the transcription of genes involved in mitogenesis and apoptosis. The p46 and p52 isoforms promote mitogenic signaling, while the p66 isoform promotes apoptosis and functions as a negative regulator of SHC1-mediated mitogenic signal transduction. Within aa 488-579, human SHC1 shares 100% aa sequence identity with mouse and rat SHC1.