Human/Mouse/Rat SHC1 Antibody

Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human, Mouse, and Rat SHC1 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human/Mouse/Rat SHC1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human SHC1 in direct ELISAs and human, mouse, and rat SHC1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human SHC3 is observed.
Monoclonal Mouse IgG1 Clone # 725001
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human SHC1
Pro379-Val470, Trp488-Val579
Accession # P29353
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat SHC1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat SHC1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat SHC1 Monoclonal Antibody (Catalog # MAB7129) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for SHC1 at approximately 66, 52, and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHC1

SH2 domain-containing transforming protein C1 (SHC1), also known as ShcA, is a cytoplasmic adaptor protein that is important in the signal transduction from growth factor, cytokine, and lymphocyte antigen receptors. SHC1 contains a PTB/PID domain (aa 156-339), a collagen homology domain (aa 340-487), and an SH2 domain (aa 488-579). Alternate splicing generates additional isoforms that differ in the extent of N-terminal truncation. Activation of SHC1 by phosphorylation at Ser239, Ser240, and Tyr317 enables Shc1 to interact with the GRB2/SOS complex, leading to the transcription of genes involved in mitogenesis and apoptosis. The p46 and p52 isoforms promote mitogenic signaling, while the p66 isoform promotes apoptosis and functions as a negative regulator of SHC1-mediated mitogenic signal transduction. Within aa 488-579, human SHC1 shares 100% aa sequence identity with mouse and rat SHC1.

Long Name
SHC-transforming Protein 1
Entrez Gene IDs
6464 (Human); 20416 (Mouse); 85385 (Rat)
Alternate Names
FLJ26504; p66; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC; SHC1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1

Product Datasheets

You must select a language.


Citation for Human/Mouse/Rat SHC1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Oncostatin M Induces Lipolysis and Suppresses Insulin Response in 3T3-L1 Adipocytes
    Authors: JL Bailey, H Hang, A Boudreau, CM Elks
    International Journal of Molecular Sciences, 2022-04-23;23(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse/Rat SHC1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat SHC1 Antibody and earn rewards!

Have you used Human/Mouse/Rat SHC1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review