Human/Mouse/Rat SHC1 Antibody

MAB7129 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7129
MAB7129-SP

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Human/Mouse/Rat SHC1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human SHC1 in direct ELISAs and human, mouse, and rat SHC1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human SHC3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 725001

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SHC1
Pro379-Val470, Trp488-Val579
Accession # P29353

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Human, Mouse, and Rat SHC1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat SHC1 Monoclonal Antibody (Catalog # MAB7129) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for SHC1 at approximately 66, 52, and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHC1

SH2 domain-containing transforming protein C1 (SHC1), also known as ShcA, is a cytoplasmic adaptor protein that is important in the signal transduction from growth factor, cytokine, and lymphocyte antigen receptors. SHC1 contains a PTB/PID domain (aa 156-339), a collagen homology domain (aa 340-487), and an SH2 domain (aa 488-579). Alternate splicing generates additional isoforms that differ in the extent of N-terminal truncation. Activation of SHC1 by phosphorylation at Ser239, Ser240, and Tyr317 enables Shc1 to interact with the GRB2/SOS complex, leading to the transcription of genes involved in mitogenesis and apoptosis. The p46 and p52 isoforms promote mitogenic signaling, while the p66 isoform promotes apoptosis and functions as a negative regulator of SHC1-mediated mitogenic signal transduction. Within aa 488-579, human SHC1 shares 100% aa sequence identity with mouse and rat SHC1.

Long Name

SHC-transforming Protein 1

Entrez Gene IDs

6464 (Human); 20416 (Mouse); 85385 (Rat)

Alternate Names

FLJ26504; p66; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC; SHC1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1