|Detection of Human SMC1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human SMC1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3294) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SMC1 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Structural Maintenance of Chromosome 1 (SMC1) is an ATPase which, with SMC3, forms a heterodimeric complex. These dimers form a V-shaped molecule with two long coiled-coil arms, each having an ATP-binding domain at its distal end. The SMC1/SMC3 heterodimer plays an important role in sister chromatid cohesion during metaphase, associates with the nuclear matrix and centrosome during interphase and localizes to kinetochores, spindles and spindle poles during mitosis. Smc1 is also a component of the DNA damage response and participates in the cellular response to DNA damage through its phosphorylation on Ser 957 and 966.
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