Human/Mouse/Rat SOD2/Mn-SOD Antibody
Human/Mouse/Rat SOD2/Mn-SOD Antibody Summary
Lys25-Lys222
Accession # P04179
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat SOD2/Mn-SOD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOD2/Mn-SOD at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
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Detection of Human and Mouse SOD2/Mn‑SOD by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD2/Mn-SOD at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOD2/Mn-SOD
Superoxide Dismutases (SODs), originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally occurring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 2 (SOD2, also known as Manganese (Mn) SOD, mitochondrial SOD and IPO-B) is an intramitochondrial 23 kDa protein that associates as a homotetramer. Each SOD2 monomer binds one Mn2+ ion. Oxidative stress has been implicated in many diseases and the chief source of reactive oxygen species within the cell is the mitochondrion. SOD2 is a free radical scavenging enzyme that protects against damage from superoxide produced as a byproduct of oxidative phosphorylation and protects the integrity of mitochondrial enzymes susceptible to inactivation by superoxide. Over aa 25‑222, human SOD2 shows 94% and 93% identity to mouse and rat SOD2.
Product Datasheets
Citations for Human/Mouse/Rat SOD2/Mn-SOD Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle
Authors: H Fujiyoshi, T Egawa, E Kurogi, T Yokokawa, K Kido, T Hayashi
International Journal of Molecular Sciences, 2022;23(3):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Mitochondrial-Targeted Decyl-Triphenylphosphonium Enhances 2-Deoxy-D-Glucose Mediated Oxidative Stress and Clonogenic Killing of Multiple Myeloma Cells
PLoS ONE, 2016;11(11):e0167323.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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