Human/Mouse/Rat Teneurin-2 Antibody

Catalog # Availability Size / Price Qty
AF4578
AF4578-SP
Human/Mouse/Rat Teneurin-2 Antibody in Western Blot
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Product Details
Citations (1)
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Human/Mouse/Rat Teneurin-2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects Teneurin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Teneurin-3 is observed, and less than 4% cross-reactivity with rhTeneurin-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Teneurin-2
Met1-Lys183
Accession # AAI72353
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot <STRONG>Detection of Mouse and Rat Teneurin-2 by Western blot.</STRONG> View Larger

Detection of Mouse and Rat Teneurin-2 by Western blot. Western blot shows lysates of mouse brain tissue and rat embryonic cortical neuron cells. PVDF Membrane was probed with 1 µg/mL of Human Teneurin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4578) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin-2 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Teneurin‑2 in Mouse Brain. View Larger

Teneurin‑2 in Mouse Brain. Teneurin-2 was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Human/Mouse/Rat Teneurin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4578) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in dorsal root ganglia and cells in cartilage primordium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Teneurin-2

Teneurin-2 (also Ten-2/Ten-m2, tenascin-M2, and Ten-m/Odz2) is a 250-330 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar Purkinje cells, pyramidal neurons of the hippocampus, and neurons of layers II and VI of the cerebral cortex. Teneurin-2 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human teneurin-2 is a 2774 amino acid (aa) type II TM glycoprotein. It contains a 379 aa cytoplasmic region (aa 1-379) that contains a polySer segment (aa 331-334), plus a 2374 aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa 575-841), five NHL repeats, each of which forms a beta -propeller (aa 1272-1573), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. There is a furin cleavage site after Arg528. There are three potential splice variants. One shows a deletion of aa 799-807, while two others show 46 and five aa substitutions for aa 1-167, respectively. Over aa 1-183, human teneurin-2 shares 98% aa identity with mouse teneurin-2.

Entrez Gene IDs
57451 (Human); 23964 (Mouse); 117242 (Rat)
Alternate Names
teneurin-2; ODZ2; Ten-2; Tenascin-M2; teneurin transmembrane protein 2; Teneurin2; Teneurin-2; Ten-m2; TNM2

Product Datasheets

Citation for Human/Mouse/Rat Teneurin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones
    Authors: NV Vysokov, JP Silva, VG Lelianova, J Suckling, J Cassidy, JK Blackburn, N Yankova, MB Djamgoz, SV Kozlov, AG Tonevitsky, YA Ushkaryov
    Elife, 2018;7(0):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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