Human/Mouse/Rat Teneurin-2 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects Teneurin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Teneurin-3 is observed, and less than 4% cross-reactivity with rhTeneurin-1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Teneurin-2
    Met1-Lys183
    Accession # AAI72353
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat Teneurin-2 by Western blot. Western blot shows lysates of mouse brain tissue and rat embryonic cortical neuron cells. PVDF Membrane was probed with 1 µg/mL of Human Teneurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4578) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin‑2 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
Teneurin‑2 in Mouse Brain. Teneurin‑2 was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Human/Mouse/Rat Teneurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4578) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in dorsal root ganglia and cells in cartilage primordium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Teneurin-2

Teneurin-2 (also Ten-2/Ten-m2, tenascin-M2, and Ten-m/Odz2) is a 250-330 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar Purkinje cells, pyramidal neurons of the hippocampus, and neurons of layers II and VI of the cerebral cortex. Teneurin-2 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human teneurin-2 is a 2774 amino acid (aa) type II TM glycoprotein. It contains a 379 aa cytoplasmic region (aa 1-379) that contains a polySer segment (aa 331-334), plus a 2374 aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa 575-841), five NHL repeats, each of which forms a beta -propeller (aa 1272-1573), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. There is a furin cleavage site after Arg528. There are three potential splice variants. One shows a deletion of aa 799-807, while two others show 46 and five aa substitutions for aa 1-167, respectively. Over aa 1-183, human teneurin-2 shares 98% aa identity with mouse teneurin-2.

  • Entrez Gene IDs:
    57451 (Human); 23964 (Mouse); 117242 (Rat)
  • Alternate Names:
    teneurin-2; ODZ2; Ten-2; Tenascin-M2; teneurin transmembrane protein 2; Teneurin2; Teneurin-2; Ten-m2; TNM2
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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