Human/Mouse/Rat TRRAP Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat TRRAP in Western blots. Detects recombinant human TRRAP in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human TRRAP
    Asn3610-Leu3859, predicted
    Accession # Q9Y4A5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat TRRAP by Western Blot.

Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, mouse placenta tissue, mouse uterus tissue, and rat ovary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TRRAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRRAP at approximately 440 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRRAP

TRRAP (Transformation/transcription domain-associated protein; also PAF350/400 and STAF40) is a 400-450 kDa member of the TRA1 subfamily, PI3/PI4 kinase family, ATM superfamily of molecules. It is a ubiquitously expressed nuclear protein that functions as a promoter of gene transcription. In particular, the activity of the E2F transcription factor family is regulated by the degree of histone acetylation. Deacetylation inhibits E2F-mediated transcription, while acetylation promotes gene transcription. TRRAP promotes histone acetylation by serving as a positive modulator/cofactor for GCN5 acetyltransferase activity. TRRAP also acts independently of GCN5. It serves as a recruiter for Skp1, a ubiquitin ligase that ubiquitates chromatin-bound beta -catenin, leading to a downregulation of Wnt-signaling activity. Human TRRAP is 3859 amino acids (aa) in length. It contains one Pro-rich region (aa 485-526), an NLS (aa 2047-2062), two FAT domains (aa 2704-3275 and 3827-3859) and a nonenzymatic PI3/PI4 kinase domain (aa 3528-3826). There are multiple potential isoform variants. They show various combinations of a deletion involving aa 1493-1510, an Ala substitution for aa 3001-3012, a 119 aa substitution for aa 3784-3859, and an 18 aa insertion after Asp3287. Over aa 3610-3859, human TRRAP shares 99% aa identity with mouse TRRAP.

  • Long Name:
    Transformation/Transcription Domain-associated Protein
  • Entrez Gene IDs:
    8295 (Human)
  • Alternate Names:
    350/400 kDa PCAF-associated factor; FLJ10671; PAF350/400; PAF400; PAF400STAF40; STAF40; Tra1 homolog; Tra1; transformation/transcription domain-associated protein; TR-AP; TRRAP
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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