Human/Mouse RBP4/Retinol-Binding Protein 4 Antibody

Catalog # Availability Size / Price Qty
MAB34761
MAB34761-SP
Detection of Mouse RBP4/Retinol‑Binding Protein 4 by Western Blot.
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Product Details
Citations (1)
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Human/Mouse RBP4/Retinol-Binding Protein 4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse RBP4/Retinol‑Binding Protein 4 in direct ELISA and Western Blot. In Western blots, detects human RBP4/Retinol‑Binding Protein 4.
Source
Monoclonal Rat IgG2A Clone # 423619
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RBP4/Retinol‑Binding Protein 4
Met1-Leu201
Accession # NP_035385
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05-2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse RBP4/Retinol-Binding Protein 4 antibody by Western Blot. View Larger

Detection of Mouse RBP4/Retinol‑Binding Protein 4 by Western Blot. Western blot shows lysates of mouse serum. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human RBP4/Retinol‑Binding Protein 4 antibody by Western Blot. View Larger

Detection of Human RBP4/Retinol‑Binding Protein 4 by Western Blot. Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 0.05 µg/mL of Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of RBP4/Retinol-Binding Protein 4 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of RBP4/Retinol-Binding Protein 4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with Retinol-binding Protein 4 (RBP4), also known as Plasma Retinol-binding Protein (1‑3). A prototypic member of the lipocalin superfamily, RBP4 has a beta ‑barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes, implying that RBP4, an adipocyte-derived signal, may be a biomarker and a drug target for the two diseases. The amino acid sequence of mouse RBP4 is 99%, 86%, 83% and 75% identical to that of rat, human/chimpanzee, dog and chicken.

References
  1. Zanotti, G. and R. Berni (2004) Vitamins and Hormones 69:271.
  2. Newcomer, M.E. and D.E. Ong (2000) Biochim. Biophys. Acta 1482:57.
  3. Yang, Q. et al. (2005) Nature 436:356.
Entrez Gene IDs
5950 (Human); 19662 (Mouse); 25703 (Rat)
Alternate Names
interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma

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Citation for Human/Mouse RBP4/Retinol-Binding Protein 4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation
    Authors: R Yentrapall, J Merl-Pham, O Azimzadeh, L Mutschelkn, C Peters, SM Hauck, MJ Atkinson, S Tapio, S Moertl
    Int. J. Radiat. Biol., 2017;0(0):1-12.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

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