Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 423619
View all RBP4/Retinol-Binding Protein 4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RBP4/Retinol‑Binding Protein 4
Met1-Leu201
Accession # NP_035385

Specificity

Detects mouse RBP4/Retinol‑Binding Protein 4 in direct ELISA and Western Blot. In Western blots, detects human RBP4/Retinol‑Binding Protein 4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse RBP4/Retinol‑Binding Protein 4 Antibody

Detection of Mouse RBP4/Retinol-Binding Protein 4 antibody by Western Blot.

Detection of Mouse RBP4/Retinol‑Binding Protein 4 by Western Blot.

Western blot shows lysates of mouse serum. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human RBP4/Retinol‑Binding Protein 4 antibody by Western Blot.

Detection of Human RBP4/Retinol‑Binding Protein 4 by Western Blot.

Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 0.05 µg/mL of Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of RBP4/Retinol-Binding Protein 4 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of RBP4/Retinol-Binding Protein 4 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761, filled histogram) or isotype control antibody (MAB006), open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Applications for Human/Mouse RBP4/Retinol‑Binding Protein 4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Western Blot

0.05-2 µg/mL
Sample: Mouse serum, human liver tissue, and human serum

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with Retinol-binding Protein 4 (RBP4), also known as Plasma Retinol-binding Protein (1‑3). A prototypic member of the lipocalin superfamily, RBP4 has a beta ‑barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes, implying that RBP4, an adipocyte-derived signal, may be a biomarker and a drug target for the two diseases. The amino acid sequence of mouse RBP4 is 99%, 86%, 83% and 75% identical to that of rat, human/chimpanzee, dog and chicken.

References

  1. Zanotti, G. and R. Berni (2004) Vitamins and Hormones 69:271.
  2. Newcomer, M.E. and D.E. Ong (2000) Biochim. Biophys. Acta 1482:57.
  3. Yang, Q. et al. (2005) Nature 436:356.

Alternate Names

RetinolBinding Protein 4

Entrez Gene IDs

5950 (Human); 19662 (Mouse); 25703 (Rat)

Gene Symbol

RBP4

UniProt

NP_035385

Additional RBP4/Retinol-Binding Protein 4 Products

Product Documents for Human/Mouse RBP4/Retinol‑Binding Protein 4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse RBP4/Retinol‑Binding Protein 4 Antibody

For research use only

Citations for Human/Mouse RBP4/Retinol‑Binding Protein 4 Antibody

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail