Human/Mouse S5a/Angiocidin Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse S5a/Angiocidin in  Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human S5a/Angiocidin
    Met1-Lys377
    Accession # P55036
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse S5a/Angiocidin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, AtT‑20 mouse pituitary tumor cell line, IMR‑32 human neuroblastoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8036) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S5a/Angiocidin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S5a/Angiocidin

S5a (26S proteasome regulatory subunit S5a/RPN10/nonATPase 4; also Angiocidin, ASF and multiubiquitin chain-binding protein) is a 50-60 kDa protein that belongs to the proteasome subunit S5A family of molecules. It is ubiquitously expressed, and found in both cytosol and in association with the 26S proteosome. Intracellularly, proteins targeted for degradation are marked by ubiquitin (Ub), a 9 kDa polypeptide. Following ubiquitination, proteins so marked are recognized by a degradation unit composed of three complexes, a central 20S complex and two flanking 19S complexes. The 20S barrel-shaped complex contains 28 subunits, one of which is S5a. This subunit binds Lys48 multiubiquitinated proteins, and contributes to proteosome-based proteolysis. S5a also binds proteins with intrinsic Ub-like sequences, and is posited to contribute to their degradation. Notably, S5a interacts with E3/E2 pairs to insure that non-forked Ub chains appear on target proteins. Failure to do so leads to forked Ub chains that are poor proteosome targets. S5a also exists in the circulation, and appears to bind TSP-1. Extracellularly, S5a is reported to possess both antiangiogenic and antiproliferative activity, and to promote inflammatory mediator secretion from mononuclear cells. Human S5a is synthesized as a 377 amino acid (aa) protein that contains one vWF-A type domain (aa 5-188) and two UIM, or Ub-interacting motifs (aa 211-230 and 282-301). There are at least three utilized Ser phosphorylation sites. Although mouse S5a has four reported splice forms, human appears to have but two, one that contains a GlyGluArg insertion after Glu254 (called angiocidin), and a second 32-33 kDa isoform that exists in the embryo and contains a 14 aa substitution for aa 255-377. Full-length human S5a shares 96% aa sequence identity with mouse S5a.

  • Long Name:
    26S Proteasome Regulatory Subunit S5a
  • Entrez Gene IDs:
    5710 (Human); 19185 (Mouse); 83499 (Rat)
  • Alternate Names:
    Angiocidin; ASF; Macropain; Mcb1; PSMD4; pUB-R5; Rpn10 ; S5a
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse S5a/Angiocidin Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information