|Detection of Human/Mouse SOD1/Cu‑Zn SOD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse SOD1/Cu‑Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD1/Cu-Zn SOD was detected at approximately 16-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
Detection of Human SOD1/Cu‑Zn SOD by Simple WesternTM. |
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu‑Zn SOD at approximately 25 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu‑Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
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Western Blot: Human/Mouse SOD1/Cu‑Zn SOD Antibody [AF3418]
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