Human/Mouse SOX2 PerCP-conjugated Antibody

IC2018C has been discontinued. View all SOX2 products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse SOX2 in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 245610
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOX2
    Gly135-Met317
    Accession # P48431
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SOX2 PerCP‑conjugated Monoclonal Antibody (Catalog # IC2018C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SOX2
SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
  • References:
    1. Graham, V. et al. (2003) Neuron 39:749.
    2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
    3. Kishi, M. et al. (2000) Development 127:791.
    4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
    5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
    6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
  • Long Name:
    Transcription Factor SOX2
  • Entrez Gene IDs:
    6657 (Human)
  • Alternate Names:
    ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2
Related Research Areas

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