Human/Mouse WASF3/WAVE3 Antibody Summary
Met1-Lys180
Accession # Q9UPY6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human WASF3/WAVE3 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, SH-SY5Y human neuroblastoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF3/WAVE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF3/WAVE3 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASF3/WAVE3
WASF3/WAVE3 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 3) is a 70 kDa member of the SCAR/WAVE family of proteins. It is expressed in select diverse cell types such as neurons, platelets, and breast epithelium. Upon activation, WASF3/WAVE3 is phosphorylated on Tyr151/248/337/486 and, in complex with HSPC300, initiates actin polymerization. WASF3/WAVE3 also regulates MMP expression. Human WASF3/WAVE3 is 502 amino acids (aa) in length. It contains one coiled-coil region (aa 57‑93), three sequential poly-Pro regions (aa 304‑410) and one VPH domain (aa 440‑457) that binds to actin and Arp2/3. There is one isoform that shows a 36 aa substitution for aa 181‑239. Over aa 1‑180, human WASF3 shares 98% aa identity with mouse WASF3.
Product Datasheets
Citations for Human/Mouse WASF3/WAVE3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex
Authors: Wang, Y;Chiappetta, G;Guérois, R;Liu, Y;Romero, S;Boesch, DJ;Krause, M;Dessalles, CA;Babataheri, A;Barakat, AI;Chen, B;Vinh, J;Polesskaya, A;Gautreau, AM;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PCARE requires coiled coil, RP62 kinase-binding and EVH1 domain-binding motifs for ciliary expansion
Authors: Tess A V Afanasyeva, Yan-Ting Schnellbach, Toby J Gibson, Ronald Roepman, Rob W J Collin
Human Molecular Genetics
Species: Mouse
Sample Types: Whole Cells
Applications: Immunocytochemistry
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