Detection of Human WASF3/WAVE3 by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line, SH‑SY5Y human neuroblastoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF3/WAVE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF3/WAVE3 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
WASF3/WAVE3 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 3) is a 70 kDa member of the SCAR/WAVE family of proteins. It is expressed in select diverse cell types such as neurons, platelets, and breast epithelium. Upon activation, WASF3/WAVE3 is phosphorylated on Tyr151/248/337/486 and, in complex with HSPC300, initiates actin polymerization. WASF3/WAVE3 also regulates MMP expression. Human WASF3/WAVE3 is 502 amino acids (aa) in length. It contains one coiled-coil region (aa 57‑93), three sequential poly-Pro regions (aa 304‑410) and one VPH domain (aa 440‑457) that binds to actin and Arp2/3. There is one isoform that shows a 36 aa substitution for aa 181‑239. Over aa 1‑180, human WASF3 shares 98% aa identity with mouse WASF3.
Wiskott-Aldrich Syndrome Protein Family Member 3
Entrez Gene IDs:
10810 (Human); 245880 (Mouse)
Brush-1; KIAA0900WASP family Verprolin-homologous protein 3; Protein WAVE-3; SCAR3; SCAR3WASP family protein member 3; Verprolin homology domain-containing protein 3; WAS protein family, member 3; WASF3; WAVE3; WAVE3Brush-1; wisk
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