Human MUC-1 Antibody

Catalog # Availability Size / Price Qty
MAB6298
MAB6298-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human MUC‑1 by Western Blot.
6 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human MUC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MUC-1 in direct ELISAs and Western blots.  In direct ELISAs, no cross-reactivity with recombinant human (rh) MUC-20, rhMUC-20S, or rhCA125/MUC-16 was observed.
Source
Monoclonal Mouse IgG2B Clone # 604804
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MUC-1
Pro126-Arg145
Accession # P15941
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
40 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MUC-1 antibody by Western Blot. View Larger

Detection of Human MUC‑1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MUC-1 at approximately 300 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of MUC-1 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MUC-1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Immunocytochemistry MUC-1 antibody in Capan-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

MUC‑1 in Capan‑1 Human Cell Line. MUC-1 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MUC-1 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

MUC‑1 in Human Breast. MUC-1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human MUC-1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human MUC‑1 by Simple WesternTM. Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for MUC‑1 at approximately 300-500 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human MUC‑1 Monoclonal Antibody (Catalog # MAB6298). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Immunocytochemistry/ Immunofluorescence Detection of Human MUC1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human MUC1 by Immunocytochemistry/Immunofluorescence Co-localization of PAM4 antigen with MUC5AC by immunofluorescence staining(A) Mucin-expressing cell lines were stained with DAPI, hPAM4, and anti-MUC5AC (2-12M1 for Capan-1 and BxPC-3; 2-11M1 for HT-29 and MCF-7), then examined by immunofluorescence microcopy. (B) BxPC-3 and HT-29 cells were stained with DAPI, hPAM4, and alpha -MUC1. PAM4 antigen was shown to co-localize with MUC5AC, not MUC1. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.2760), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUC-1

MUC-1 (Mucin-1; also PEM, PEMT, Episialin, tumor-associated mucin, and carcinoma associated mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O-glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.

Long Name
Mucin 1, Cell Surface-associated
Entrez Gene IDs
4582 (Human)
Alternate Names
Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

Product Datasheets

You must select a language.

x

Citation for Human MUC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
    Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
    Cancer Res., 2017-04-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human MUC-1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human MUC-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human MUC-1 Antibody
By Christian Ostalecki on 11/12/2021
Application: IHC Sample Tested: Skin cancer (squamos cell) Species: Human