Human MUC-1 Antibody
Human MUC-1 Antibody Summary
Pro126-Arg145
Accession # P15941
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MUC‑1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MUC-1 at approximately 300 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
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Detection of MUC-1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
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MUC‑1 in Capan‑1 Human Cell Line. MUC-1 was detected in immersion fixed Capan-1 human pancreatic adenocarcinoma cell line using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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MUC‑1 in Human Breast. MUC-1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB6298) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human MUC‑1 by Simple WesternTM. Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for MUC‑1 at approximately 300-500 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human MUC‑1 Monoclonal Antibody (Catalog # MAB6298). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MUC-1
MUC-1 (Mucin-1; also PEM, PEMT, Episialin, tumor-associated mucin, and carcinoma associated mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O-glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.
Product Datasheets
Citation for Human MUC-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
Cancer Res., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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