MyD88 in Raji Human Cell Line. MyD88 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human MyD88 Monoclonal Antibody (Catalog # MAB2928) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MyD88 was first identified as a myeloid differentiation primary response protein. It contains a cytoplasmic Toll/interleukin 1 receptor (TIR) domain that classifies it as a member of the TIR superfamily. MyD88 is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.
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