Human/Primate NCAM-1/CD56 Antibody

(1 citations)   
  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human NCAM‑1/CD56 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM-1-L1, recombinant mouse (rm) MAdCAM-1, rmNCAM-1-L1, or rmOCAM is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 301040
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NCAM‑1/CD56
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    Human peripheral blood mononuclear cells
    • Human/Primate NCAM-1/CD56 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Primate NCAM‑1/CD56 Antibody (Catalog # MAB2408)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate NCAM‑1/CD56 Biotinylated Antibody (Catalog # BAF2408)
  • ELISA Standard
     
    Recombinant Human NCAM-1/CD56 Protein, CF (Catalog # 2408-NC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human NCAM‑1/CD56 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Primate NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB2408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).

  • References:
    1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
    2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
    3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
    4. Dickson, G. et al. (1987) Cell 50:1119.
    5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
    6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
    7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
    8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
    9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
    10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
    11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.
    12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
    13. Muller, D. et al. (1996) Neuron 17:413.
  • Long Name:
    Neural Cell Adhesion Molecule
  • Entrez Gene IDs:
    4684 (Human); 17967 (Mouse); 24586 (Rat)
  • Alternate Names:
    CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAM-1/CD56; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Natural killer cell killing of acute myelogenous leukemia and acute lymphoblastic leukemia blasts by killer cell immunoglobulin-like receptor-negative natural killer cells after NKG2A and LIR-1 blockade.
    Authors: Godal R, Bachanova V, Gleason M
    Biol. Blood Marrow Transplant., 2010;16(5):612-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
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Mouse IgG2B Isotype Control

Ctrl MAB004 22  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 5
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Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 16  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NL557 Affinity Purified PAb

Flow, IHC, ICC NL007 8
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
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