Human Neprilysin/CD10 APC-conjugated Antibody

FAB8160A has been discontinued. View all Neprilysin/CD10 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Neprilysin/CD10 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant mouse Neprilysin is observed, and less than 0.3% cross-reactivity with recombinant human Neprilysin-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
    Tyr52-Trp750
    Accession # P08473
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Neprilysin/CD10 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Goat Anti-Human Neprilysin/CD10 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8160A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Neprilysin/CD10 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Goat Anti-Human Neprilysin/CD10 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8160A) or (B) Normal Goat IgG Allophycocyanin Control (Catalog # IC108A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Neprilysin/CD10

Neprilysin/CD10, also known as NEP and neutral endopeptidase 24.11, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta  peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (5).

  • References:
    1. Malfroy, B. et al. (1978) Nature 276:523.
    2. Kenny, A.J. and Stephenson, S.L. (1988) FEBS Lett. 232:1.
    3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
    4. Shipp, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4819.
    5. Itwata, N. et al. (2001) Science 292:1550.
     
  • Entrez Gene IDs:
    4311 (Human); 17380 (Mouse); 24590 (Rat)
  • Alternate Names:
    Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase
Related Research Areas

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