Human Nesfatin-1/Nucleobindin-2 Antibody (877140)

R&D Systems | Catalog # MAB5949

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 877140
View all Nesfatin-1/Nucleobindin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nesfatin-1/Nucleobindin-2
Val25-Leu106
Accession # P80303

Specificity

Detects human Nesfatin-1/Nucleobindin-2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Nesfatin-1/Nucleobindin-2 Antibody (877140)

Detection of Human Nesfatin-1/Nucleobindin-2 antibody by Western Blot.

Detection of Human Nesfatin‑1/Nucleobindin‑2 by Western Blot.

Western blot shows lysates of AGS human gastric adenocarcinoma cell line and human testis tissue. PVDF membrane was probed with 3 µg/mL of Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Nesfatin-1/ Nucleobindin-2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Nesfatin-1/Nucleobindin-2 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

Nesfatin‑1/Nucleobindin‑2 in Human Mesenchymal Stem Cells.

Nesfatin-1/Nucleobindin-2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Nesfatin-1/Nucleobindin-2 antibody in Human Stomach by Immunohistochemistry (IHC-P).

Nesfatin‑1/Nucleobindin‑2 in Human Stomach.

Nesfatin-1/Nucleobindin-2 was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human Nesfatin-1/ Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Nesfatin-1/Nucleobindin-2 Antibody (877140)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into adipocytes

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Western Blot

3 µg/mL
Sample: AGS human gastric adenocarcinoma cell line and human testis tissue

Reviewed Applications

Read 1 review rated 5 using MAB5949 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nesfatin-1/Nucleobindin-2

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1‑24), Nesfatin-1 (aa 25‑106), a DNA-binding site (aa 171‑223), and two EF-hand regions (aa 241‑276 and 293‑328). Intracellular uncleaved NUCB2 is 45‑50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109‑187) and Nesfatin-3 (aa 190‑420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Alternate Names

NEFA, Nesfatin1, NUCB2, Nucleobindin-2

Entrez Gene IDs

4925 (Human); 53322 (Mouse); 59295 (Rat)

Gene Symbol

NUCB2

UniProt

P80303 (Human)

Additional Nesfatin-1/Nucleobindin-2 Products

Product Documents for Human Nesfatin-1/Nucleobindin-2 Antibody (877140)

Certificate of Analysis

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Product Specific Notices for Human Nesfatin-1/Nucleobindin-2 Antibody (877140)

For research use only

Citations for Human Nesfatin-1/Nucleobindin-2 Antibody (877140)

Customer Reviews for Human Nesfatin-1/Nucleobindin-2 Antibody (877140) (1)

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  • Human Nesfatin-1/Nucleobindin-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate tissue
    Species: Human
    Verified Customer | Posted 10/28/2021
    Human Nesfatin-1/Nucleobindin-2 Antibody (877140) MAB5949

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