NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma delta T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.
Human NKG2D/CD314 Alexa Fluor® 700‑conjugated Antibody
R&D Systems | Catalog # FAB1391N
Key Product Details
Validated by
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Phe78-Val216
Accession # P26718
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human NKG2D/CD314 Alexa Fluor® 700‑conjugated Antibody
Detection of NKG2D/CD314 in NK cells by Flow Cytometry.
NK cells (PBMC treated with 27 ng/ml Recombinant Human IL-2 Protein (202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10 ng/ml Recombinant Human IL-21 Protein (8879-IL) for 15 days) were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Rabbit Anti-Human NKG2D/CD314 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1391N) or (B) Mouse IgG2B Isotype Control (Catalog # MAB004). View our protocol for Staining Membrane-associated Proteins.Detection of NKG2D/CD314 in PBMCs by Flow Cytometry.
PBMCs were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Rabbit Anti-Human NKG2D/CD314 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1391N) or (B) Rabbit IgG Alexa Fluor® 700-conjugated Antibody (Catalog # IC1051N). View our protocol for Staining Membrane-associated Proteins.Applications for Human NKG2D/CD314 Alexa Fluor® 700‑conjugated Antibody
Flow Cytometry
Spectra Viewer
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- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2D/CD314
References
- Li, P. et al. (2001) Nature Immunol. 2:443.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Cosman, D. et al. (2001) Immunity 14:123.
- Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
- Wu, J. et al. (1999) Science 285:730.
- Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
- Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
- Groh, V. et al. (2001) Nature Immunol. 2:255.
- Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
- Diefenbach, A. et al. (2001) Nature 413:165.
- NKG2D and its Ligands (2002) www.rndsystems.com
Long Name
Alternate Names
Gene Symbol
UniProt
Additional NKG2D/CD314 Products
Product Documents for Human NKG2D/CD314 Alexa Fluor® 700‑conjugated Antibody
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Product Specific Notices for Human NKG2D/CD314 Alexa Fluor® 700‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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