Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 253415
View all NKp44/NCR2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2
Gln22-Pro190
Accession # CAB52289

Specificity

Detects human NKp44/NCR2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp46, rhNKp80, or recombinant mouse NKp46 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NKp44/NCR2 APC‑conjugated Antibody

Detection of NKp44/NCR2 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of NKp44/NCR2 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and Mouse Anti-Human NKp44/NCR2 APC-conjugated Monoclonal Antibody (Catalog # FAB22491A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NKp44/NCR2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL)

Reviewed Applications

Read 2 reviews rated 5 using FAB22491A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKp44/NCR2

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

References

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
  5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.

Long Name

NK Cell Activating Receptor

Alternate Names

CD336, LY95, NCR2

Entrez Gene IDs

9436 (Human); 102129442 (Cynomolgus Monkey)

Gene Symbol

NCR2

UniProt

CAB52289

Additional NKp44/NCR2 Products

Product Documents for Human NKp44/NCR2 APC‑conjugated Antibody

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Product Specific Notices for Human NKp44/NCR2 APC‑conjugated Antibody

For research use only

Citations for Human NKp44/NCR2 APC‑conjugated Antibody

Customer Reviews for Human NKp44/NCR2 APC‑conjugated Antibody (2)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22084431
    Species: Human
    Verified Customer | Posted 01/22/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22084431
    Species: Human
    Verified Customer | Posted 01/22/2015

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