Human NKp44/NCR2 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NKp44/NCR2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp46, rhNKp80, or recombinant mouse NKp46 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 253415
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2
    Gln22-Pro190
    Accession # CAB52289
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human CD56+ peripheral blood mononuclear cells treated with Recombinant Human IL‑2 (Catalog # 202-IL)
  • Agonist Activity
    0.05-0.2 µg/mL
    See below
  • CyTOF-reported
    Gaudilliere, B. et al. (2014) Sci. Transl. Med. 6: 255ra131. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Human NKp44/NCR2 Antibody Induces IFN-gamma  Secretion in Activated Human NK Cells.

Mouse Anti-Human NKp44/NCR2 Monoclonal Antibody (Catalog # MAB22491) induces IFN-gamma secretion in human natural killer (NK) cells activated with 500 ng/mL Recombinant Human IL-2 (Catalog # 202-IL), in a dose-dependent manner, as measured using the Quantikine Human IFN-gamma ELISA Kit (Catalog # DIF50). The ED50 for this effect is typically 0.05‑0.2 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp44/NCR2

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
    2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
    3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
    4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
    5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  • Long Name:
    NK Cell Activating Receptor
  • Entrez Gene IDs:
    9436 (Human)
  • Alternate Names:
    CD336 antigen; CD336; LY95; LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44); lymphocyte antigen 95 (activating NK-receptor; NK-p44); Lymphocyte antigen 95 homolog; natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; NCR2; NK cell activating receptor (NKp44); NK cell-activating receptor; NKp44; NK-p44dJ149M18.1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Structural Insights into the Inhibitory Mechanism of an Antibody against B7-H6, a Stress-Induced Cellular Ligand for the Natural Killer Cell Receptor NKp30
    J Mol Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Regulation of NK92-MI cell cytotoxicity by substance P.
    Authors: Fu WX, Qin B, Zhou AP, Yu QY, Huang QJ, Liang ZF
    Scand. J. Immunol., 2011;74(2):107-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. NKp44 expression, phylogenesis and function in non-human primate NK cells.
    Authors: De Maria A, Ugolotti E, Rutjens E, Mazza S, Radic L, Faravelli A, Koopman G, Di Marco E, Costa P, Ensoli B, Cafaro A, Mingari MC, Moretta L, Heeney J, Biassoni R
    Int. Immunol., 2009;21(3):245-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  4. NKp44 receptor mediates interaction of the envelope glycoproteins from the West Nile and dengue viruses with NK cells.
    Authors: Hershkovitz O, Rosental B, Rosenberg LA, Navarro-Sanchez ME, Jivov S, Zilka A, Gershoni-Yahalom O, Brient-Litzler E, Bedouelle H, Ho JW, Campbell KS, Rager-Zisman B, Despres P, Porgador A
    J. Immunol., 2009;183(4):2610-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  5. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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