Human NLRP1/NALP1 Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human NLRP1/NALP1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) NALP2 and rhNALP3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human NLRP1/NALP1
    Met1-Asp323 (Leu155His)
    Accession # Q9C000
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human NLRP1/NALP1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with human NLRP1/NALP1 or transfected with NALP3, THP‑1 human acute monocytic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human NLRP1/NALP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6788) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NLRP1/NALP1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP1/NALP1

NALP1 (NAcht, Leucine-rich repeat and PYD domain containing protein 1; also NAC, CARD7, DEFCAP and CLR17.1) is a 160-170 kDa member of the NLRP family of molecules. It is expressed in dendritic cells, neutrophils, T and B cells, alveolar macrophages, permatogonia, neurons, plus intestinal columnar epithelium, and is found in both cytoplasm and nucleus. NALP1 promotes apoptosis plus IL-1 beta and IL-18 maturation by activating caspase-1 and -5. It does so by forming a 700 kDa inflammasome comprised of NALP1, ASC plus caspase-1 and -5. Bacterial wall peptidoglycan binds to NALP1, promoting ATP binding, NALP1 oligomerization, and caspase activation. Human NALP1 is 1473 amino acids (aa) in length. It contains an N-terminal DAPIN domain (aa 1-92), a NACHT domain (aa 328-637), seven consecutive LRRs (aa 704-1236) and one CARD region (aa 1374-1463). Alternate splice forms exist that range in size from 70 kDa-150 kDa. Either individually, or in combination, there can be a 19 aa substitution for either aa 1353-1473 or aa 1368-1472, a deletion of aa 91-260, 958-987 or 1262-1305, and a 43 aa insert after Leu785. Over aa 1-323, human NALP1 shares less that 20% aa identity with mouse NALP1.

  • Long Name:
    NLR Family, Pyrin Domain Containing 1
  • Entrez Gene IDs:
    22861 (Human); 195046 (Mouse); 360557 (Rat)
  • Alternate Names:
    CARD7; CARD7SLEV1; Caspase recruitment domain-containing protein 7; CLR17.1; Death effector filament-forming ced-4-like apoptosis protein; DEFCAP; DEFCAPVAMAS1; DKFZp586O1822; KIAA0926DEFCAP-L/S; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; NACHT, leucine rich repeat and PYD containing 1; NACHT, LRR and PYD containing protein 1; NACHT, LRR and PYD domains-containing protein 1; NACPP1044; NALP1; NALP1caspase recruitment domain protein 7; NLR family, pyrin domain containing 1; NLRP1; Nucleotide-binding domain and caspase recruitment domain; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1; PP1044; SLEV1; VAMAS1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress.
    Authors: D'Osualdo A, Anania V, Yu K, Lill J, Kaufman R, Matsuzawa S, Reed J
    PLoS ONE, 2015;10(6):e0130635.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

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 ELISA Human Anonymous 09/07/2017
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Summary

ApplicationELISA
Sample TestedHeLa human cervical epithelial carcinoma cell line
SpeciesHuman

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