Human NLRP1/NALP1 Antibody

Name: Human NLRP1/NALP1 Antibody
Brand: R&D Systems
SKU: AF6788-SP
Rating: 5 (1 reviews)

Catalog #: AF6788
6 Citations 1 Review Datasheet / COA / SDS

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AF6788
AF6788-SP

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Detection of Human NLRP1/NALP1 by Western Blot.

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Citations (6)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NLRP1/NALP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NLRP1/NALP1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) NALP2 and rhNALP3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NLRP1/NALP1
Met1-Asp323 (Leu155His)
Accession # Q9C000

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NLRP1/NALP1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with human NLRP1/NALP1 or transfected with NALP3, THP-1 human acute monocytic leukemia cell line, and Ramos human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human NLRP1/NALP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6788) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NLRP1/NALP1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP1/NALP1

NALP1 (NAcht, Leucine-rich repeat and PYD domain containing protein 1; also NAC, CARD7, DEFCAP and CLR17.1) is a 160-170 kDa member of the NLRP family of molecules. It is expressed in dendritic cells, neutrophils, T and B cells, alveolar macrophages, permatogonia, neurons, plus intestinal columnar epithelium, and is found in both cytoplasm and nucleus. NALP1 promotes apoptosis plus IL-1 beta and IL-18 maturation by activating caspase-1 and -5. It does so by forming a 700 kDa inflammasome comprised of NALP1, ASC plus caspase-1 and -5. Bacterial wall peptidoglycan binds to NALP1, promoting ATP binding, NALP1 oligomerization, and caspase activation. Human NALP1 is 1473 amino acids (aa) in length. It contains an N-terminal DAPIN domain (aa 1-92), a NACHT domain (aa 328-637), seven consecutive LRRs (aa 704-1236) and one CARD region (aa 1374-1463). Alternate splice forms exist that range in size from 70 kDa-150 kDa. Either individually, or in combination, there can be a 19 aa substitution for either aa 1353-1473 or aa 1368-1472, a deletion of aa 91-260, 958-987 or 1262-1305, and a 43 aa insert after Leu785. Over aa 1-323, human NALP1 shares less that 20% aa identity with mouse NALP1.

Long Name

NLR Family, Pyrin Domain Containing 1

Entrez Gene IDs

22861 (Human); 195046 (Mouse); 360557 (Rat)

Alternate Names

CARD7; CARD7SLEV1; Caspase recruitment domain-containing protein 7; CLR17.1; Death effector filament-forming ced-4-like apoptosis protein; DEFCAP; DEFCAPVAMAS1; DKFZp586O1822; KIAA0926DEFCAP-L/S; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; NACHT, leucine rich repeat and PYD containing 1; NACHT, LRR and PYD containing protein 1; NACHT, LRR and PYD domains-containing protein 1; NACPP1044; NALP1; NALP1caspase recruitment domain protein 7; NLR family, pyrin domain containing 1; NLRP1; Nucleotide-binding domain and caspase recruitment domain; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1; PP1044; SLEV1; VAMAS1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human NLRP1/NALP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome
Authors: SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin
Nature Chemical Biology, 2022;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Synergistic activation of p53 by actinomycin D and nutlin-3a is associated with the upregulation of crucial regulators and effectors of innate immunity
Authors: M Krze?niak, A Zajkowicz, A Gdowicz-K?, M G?owala-Ko, B ?asut-Szys, M Rusin
Cell. Signal., 2020;69(0):109552.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot
DPP8/9 inhibitors activate the CARD8 inflammasome in resting lymphocytes
Authors: DC Johnson, MC Okondo, EL Orth, SD Rao, HC Huang, DP Ball, DA Bachovchin
Cell Death Dis, 2020;11(8):628.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Homozygous NLRP1 gain-of-function mutation in siblings with a syndromic form of recurrent respiratory papillomatosis
Authors: SB Drutman, F Haerynck, FL Zhong, D Hum, NJ Hernandez, S Belkaya, F Rapaport, SJ de Jong, D Creytens, SJ Tavernier, K Bonte, S De Scheppe, J van der We, L Lorenzo-Di, A Wullaert, X Bossuyt, G Orth, VR Bonagura, V Béziat, L Abel, E Jouanguy, B Reversade, J Laurent-Ca
Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia
Authors: DC Johnson, CY Taabazuing, MC Okondo, AJ Chui, SD Rao, FC Brown, C Reed, E Peguero, E de Stanchi, A Kentsis, DA Bachovchin
Nat. Med., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress.
Authors: D'Osualdo A, Anania V, Yu K, Lill J, Kaufman R, Matsuzawa S, Reed J
PLoS ONE, 2015;10(6):e0130635.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation