Human NOD1 Antibody

Name: Human NOD1 Antibody
Brand: R&D Systems
SKU: AF7090

Catalog #: AF7090 Datasheet / COA / SDS

Newer Version Available: MAB7090

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Discontinued Product

AF7090 has been discontinued and is replaced by MAB7090.

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Human NOD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NOD1.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NOD1
Lys830-Phe953
Accession # Q9Y239

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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NOD1 in Mouse Skeletal Muscle. NOD1 was detected in immersion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Human NOD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7090) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NOD1

NOD1, also called CARD4, is a 108 kDa intracellular pattern recognition receptor of the Nod-like receptor (NLR or Caterpiller) family. This innate immune receptor recognizes the muramyl peptide, diaminopimelic acid. The 953 amino acid (aa) NOD1 contains a caspase recognition domain (CARD), an NTPase domain and 10 leucine‑rich repeats (LRR). Splicing variants of 925 and 779 aa lack all or part of LRR 7-10 and do not respond to muramyl peptides. Polymorphisms of NOD1 are associated with inflammatory bowel disease. Within aa 830-953 (LRR 7-110), human NOD1 shares 82% and 84% aa identity with mouse and rat NOD1, respectively.

Long Name

Nucleotide-binding Oligomerization Domain-containing Protein 1

Entrez Gene IDs

10392 (Human); 107607 (Mouse); 500133 (Rat)

Alternate Names

CARD4; CARD4NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; Caspase recruitment domain-containing protein 4; CLR7.1; NLRC1; NOD1; nucleotide-binding oligomerization domain containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 1; nucleotide-binding oligomerization domain-containing protein 1