Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 626919
View all NOD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NOD1
Lys830-Phe953
Accession # Q9Y239

Specificity

Detects human NOD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human NOD2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NOD1 Antibody

Detection of Human NOD1 antibody by Western Blot.

Detection of Human NOD1 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human NOD1 or human NOD2, HT-29 human colon adenocarcinoma cell line, and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NOD1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

NOD1 antibody in Human Spleen by Immunohistochemistry (IHC-P).

NOD1 in Human Spleen.

NOD1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human NOD1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human NOD1 or human NOD2, HT‑29 human colon adenocarcinoma cell line, and SW480 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NOD1

NOD1, also called CARD4, is a 108 kDa intracellular pattern recognition receptor of the Nod-like receptor (NLR or Caterpiller) family. This innate immune receptor recognizes the muramyl peptide, diaminopimelic acid. The 953 amino acid (aa) NOD1 contains a caspase recognition domain (CARD), an NTPase domain and 10 leucine‑rich repeats (LRR). Splicing variants of 925 and 779 aa lack all or part of LRR 7-10 and do not respond to muramyl peptides. Polymorphisms of NOD1 are associated with inflammatory bowel disease. Within aa 830-953 (LRR 7-110), human NOD1 shares 82% and 84% aa identity with mouse and rat NOD1, respectively.

Long Name

Nucleotide-binding Oligomerization Domain-containing Protein 1

Alternate Names

CARD4, CLR7.1, NLRC1

Entrez Gene IDs

10392 (Human); 107607 (Mouse); 500133 (Rat)

Gene Symbol

NOD1

UniProt

Q9Y239

Additional NOD1 Products

Product Documents for Human NOD1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NOD1 Antibody

For research use only

Citations for Human NOD1 Antibody

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Protocols

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