Human Notch-2 Intracellular Domain Biotinylated Antibody

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Human Notch-2 Intracellular Domain Biotinylated Antibody Summary

Species Reactivity
Detects human Notch-2 Intracellular Domain in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Notch‑2 Intracellular Domain
Accession # Q04721
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Notch‑2 Intracellular Domain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-2

Human Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1‑3). Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, showing that not all functions overlap (4, 5). Mice with hypomorphic Notch-2 show defects in the development of kidney, heart and eyes (6). Notch-2 is up‑regulated in mature B cells and is critical for differentiation to splenic marginal zone B cells (7). Notch-2 is also preferentially expressed in choroid plexus epithelia and neuronal precursors (8, 9). Human Notch-2 is synthesized as a 2471 amino acid (aa) precursor that contains a 25 aa signal sequence, a 1652 aa extracellular domain (ECD), and a 794 aa transmembrane (TM) and cytoplasmic segment. The ECD contains 35 EGF-like repeats and three Lin-12/Notch repeats, while the cytoplasmic region shows six ankyrin repeats, a glutamine-rich domain and a PEST sequence. Binding of ligands, including Jagged and Delta-like molecules in humans, has been localized to the 11th and 12th EGF-like repeats of Notch (10). Notch receptors undergo post-translational furin-type proteolytic cleavage (11). This forms a heterodimer through the interaction of a hydrophobic area in the ligand‑binding extracellular region with the TM/cytoplasmic portion (11, 12). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM‑17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (13). Human Notch-2 ECD (aa 26‑530) shows 93%, 93%, 96% and 96% aa identity with the corresponding regions of mouse, rat, canine, and bovine Notch-2, respectively. This region also exhibits approximately 60% aa identity with human Notch-1 and Notch-3.

  1. Stifani, S. et al. (1992) Nat. Genet. 2:119.
  2. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
  3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
  4. Hamada, Y. et al. (1999) Development 126:3415.
  5. Shimizu, K. et al. (2002) Biochem. Biophys. Res. Comm. 291:775.
  6. McCright, B. et al. (2001) Development 128:491.
  7. Saito, T. et al. (2003) Immunity 18:675.
  8. Irvin, D. K. et al. (2001) J. Comp. Neurol. 436:167.
  9. Solecki, D. J. et al. (2001) Neuron 31:557.
  10. Hambleton, S. et al. (2004) Structure 12:2173.
  11. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
  12. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
  13. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Entrez Gene IDs
4853 (Human); 29492 (Rat)
Alternate Names
AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2

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