Human Notch-2 Intracellular Domain Antibody

Catalog # Availability Size / Price Qty
AF3735
AF3735-SP
Product Details
Citations (3)
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Human Notch-2 Intracellular Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human Notch-2 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) Notch-3 (aa 2195‑2321), rhNotch-6 (aa 2251‑2556) and rhNotch-4 (aa 1778‑2003) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Notch‑2
Asp2063-Pro2413
Accession # Q04721
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Notch‑2 Intracellular Domain
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-2

Human Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1‑3). Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, showing that not all functions overlap (4, 5). Mice with hypomorphic Notch-2 show defects in the development of kidney, heart and eyes (6). Notch-2 is up‑regulated in mature B cells and is critical for differentiation to splenic marginal zone B cells (7). Notch-2 is also preferentially expressed in choroid plexus epithelia and neuronal precursors (8, 9). Human Notch-2 is synthesized as a 2471 amino acid (aa) precursor that contains a 25 aa signal sequence, a 1652 aa extracellular domain (ECD), and a 794 aa transmembrane (TM) and cytoplasmic segment. The ECD contains 35 EGF-like repeats and three Lin-12/Notch repeats, while the cytoplasmic region shows six ankyrin repeats, a glutamine-rich domain and a PEST sequence. Binding of ligands, including Jagged and Delta-like molecules in humans, has been localized to the 11th and 12th EGF-like repeats of Notch (10). Notch receptors undergo post-translational furin-type proteolytic cleavage (11). This forms a heterodimer through the interaction of a hydrophobic area in the ligand‑binding extracellular region with the TM/cytoplasmic portion (11, 12). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM‑17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (13). Human Notch-2 ECD (aa 26‑530) shows 93%, 93%, 96% and 96% aa identity with the corresponding regions of mouse, rat, canine, and bovine Notch-2, respectively. This region also exhibits approximately 60% aa identity with human Notch-1 and Notch-3.

References
  1. Stifani, S. et al. (1992) Nat. Genet. 2:119.
  2. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
  3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
  4. Hamada, Y. et al. (1999) Development 126:3415.
  5. Shimizu, K. et al. (2002) Biochem. Biophys. Res. Comm. 291:775.
  6. McCright, B. et al. (2001) Development 128:491.
  7. Saito, T. et al. (2003) Immunity 18:675.
  8. Irvin, D. K. et al. (2001) J. Comp. Neurol. 436:167.
  9. Solecki, D. J. et al. (2001) Neuron 31:557.
  10. Hambleton, S. et al. (2004) Structure 12:2173.
  11. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
  12. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
  13. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Entrez Gene IDs
4853 (Human); 29492 (Rat)
Alternate Names
AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2

Product Datasheets

Citations for Human Notch-2 Intracellular Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. NOTCH2 negatively regulates metastasis and epithelial-Mesenchymal transition via TRAF6/AKT in nasopharyngeal carcinoma
    Authors: Y Zou, R Yang, ML Huang, YG Kong, JF Sheng, ZZ Tao, L Gao, SM Chen
    J. Exp. Clin. Cancer Res., 2019;38(1):456.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Involvement of Notch in activation and effector functions of gammadelta T cells.
    Authors: Gogoi D, Dar A, Chiplunkar S
    J Immunol, 2014;192(5):2054-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
    Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
    Br. J. Dermatol., 2011;164(6):1228-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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