Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Numb Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Numb
Thr356-Leu592
Accession # AAH68476

Specificity

Detects human Numb in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Numb Antibody

Detection of Human Numb antibody by Western Blot.

Detection of Human Numb by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Numb Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4338) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Numb at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Numb in RT‑4 Human Cell Line.

Numb was detected in immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line using Sheep Anti-Human Numb Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4338) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Numb Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Numb

Numb is a member of a family of cytoplasmic adaptor proteins termed CLASPS (Clathran-associated Sorting Proteins). These link transmembrane cargo proteins to the endocytic machinery. Numb, a conserved cell-fate determining factor, is normally associated with the basolateral surface of polarized cells. Movement towards the apical surface induces its phosphorylation, which precludes its entry into this region. Human Numb is 651 amino acids (aa) in length. It contains one PTB (aa 24‑172) and one PRR (aa 366‑413) domain. There is a potential for multiple alternate splice forms that involve all regions of the molecule. Over aa 356‑592, human Numb shares 88% and 90% aa identity with mouse and canine Numb, respectively.

Alternate Names

m-numb, mnb, S171

Entrez Gene IDs

8650 (Human); 18222 (Mouse); 29419 (Rat)

Gene Symbol

NUMB

UniProt

AAH68476

Additional Numb Products

Product Documents for Human Numb Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Numb Antibody

For research use only

Related Research Areas

Neural Crest Cell Markers
Notch Pathway

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Protocols

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