Detection of Human Numb by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Numb Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4338) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Numb at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Numb is a 75 kDa member of a family of cytoplasmic adaptor proteins termed CLASPS (clathrin-associated sorting proteins). These link transmembrane cargo proteins to the endocytic machinery. Numb is normally associated with the basolateral surface of polarized cells and has been shown to regulate cell fate by modulating activity of the Notch pathway. Human Numb is 651 amino acids (aa) in length. It contains one phosphotyrosine-binding domain (PTB; aa 24‑172) and one proline-rich region (PRR; aa 366‑413). There is a potential for multiple alternate splice forms that involve all regions of the molecule. Over aa 356‑592, human Numb shares 88% and 90% aa identity with mouse and canine Numb, respectively.
Entrez Gene IDs:
8650 (Human); 18222 (Mouse); 29419 (Rat)
h-Numb; mnb; m-numb; numb (Drosophila) homolog; numb homolog (Drosophila); Numb; protein numb homolog; Protein S171; S171
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