Human OLT-2/TARM1 Antibody Summary
Accession # B6A8C7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of OLT-2/TARM1 in Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (Catalog # MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.
Detection of OLT-2/TARM1 on U937 Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Rabbit anti-Human OLT-2/TARM1 Monoclonal Antibody (MAB10059, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Phycoerythrin-conjugated Secondary Antibody (F0110). Staining was performed using our Staining Membrane-Associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4+ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).
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