Human Oncostatin M/OSM Antibody

Catalog # Availability Size / Price Qty
MAB295-SP
MAB295-100
MAB295-500
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human Oncostatin M/OSM Antibody.
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Product Details
Citations (10)
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Reviews (1)

Human Oncostatin M/OSM Antibody Summary

Species Reactivity
Human
Specificity
Detects human Oncostatin M in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-6, rhIL-11, rhCNTF, or rhLIF.
Source
Monoclonal Mouse IgG2A Clone # 17001
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human Oncostatin M/OSM
Ala26-Arg234
Accession # P13725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Oncostatin M/OSM (Catalog # 295-OM)

Human Oncostatin M/OSM Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Oncostatin M/OSM Biotinylated Antibody (Catalog # BAF295)

Standard: Recombinant Human Oncostatin M (OSM) Protein (Catalog # 295-OM)

Neutralization
Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 2 ng/mL Recombinant Human Oncostatin M/OSM.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human Oncostatin M/OSM Antibody. View Larger

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human Oncostatin M/OSM Antibody. Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M/OSM (2 ng/mL) is neutralized (green line) by increasing concentrations of Human Oncostatin M/OSM Monoclonal Antibody (Catalog # MAB295). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oncostatin M/OSM

OSM is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.

 

OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. The gp130, a signal transducing component ( beta  subunit) of the IL-6, LIF and CNTF receptor complexes, was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130-related protein) has now been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Since OSM is also active on cells that do not express LIF R, a specific OSM receptor that does not involve LIF R must also exist. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDS-Kaposi’s sarcoma cells, and a human erythroleukemia cell line, TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells; regulation of IL-6 expression in human endothelial cells; and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells.

Entrez Gene IDs
5008 (Human); 18413 (Mouse)
Alternate Names
MGC20461; oncostatin M; oncostatin-M; OSM

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Citations for Human Oncostatin M/OSM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Bone Marrow Macrophages Contribute to Diabetic Stem Cell Mobilopathy by Producing Oncostatin M
    Authors: Mattia Albiero, Nicol Poncina, Stefano Ciciliot, Roberta Cappellari, Lisa Menegazzo, Francesca Ferraro et al.
    Diabetes
  2. Oncostatin M-Enriched Small Extracellular Vesicles Derived from Mesenchymal Stem Cells Prevent Isoproterenol-Induced Fibrosis and Enhance Angiogenesis
    Authors: Sandra Tejedor, Marc Buigues, Hernán González-King, Andreia M. Silva, Nahuel Aquiles García, Niek Dekker et al.
    International Journal of Molecular Sciences
  3. Treponema denticola stimulates Oncostatin M cytokine release and de novo synthesis in neutrophils and macrophages
    Authors: MM Jones, ST Vanyo, W Ibraheem, A Maddi, MB Visser
    J. Leukoc. Biol., 2020-07-17;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies
    Authors: C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan
    J. Leukoc. Biol., 2018-07-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Macrophage-derived oncostatin M contributes to human and mouse neurogenic heterotopic ossifications
    Authors: F Torossian, B Guerton, A Anginot, KA Alexander, C Desterke, S Soave, HW Tseng, N Arouche, L Boutin, I Kulina, M Salga, B Jose, AR Pettit, D Clay, N Rochet, E Vlachos, G Genet, C Debaud, P Denormandi, F Genet, NA Sims, S Banzet, JP Levesque, JJ Lataillade, MC Le Bousse-
    JCI Insight, 2017-11-02;2(21):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy.
    Authors: Su C, Chiang Y, Huang C, Hsu C, Fong Y, Tang C
    J Immunol, 2015-08-24;195(7):3355-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012-07-03;7(7):e39871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
    Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
    J. Biol. Chem., 2011-07-22;286(39):34271-85.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  9. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  10. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Human Oncostatin M/OSM Antibody
By Anonymous on 10/07/2021
Application: WB Sample Tested: TF-1 human erythroleukemic cell line Species: Human