ONECUT2 (one "cut" domain family member 2; also OC-2 and HNF6 beta ) is a monomeric 54 kDa (predicted) member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic neural crest plus hepatic, pancreatic, and intestinal tissue. In the adult, it is found in hepatocytes and small intestine epithelial cells. ONECUT2 is a transcriptional regulator that is known to impact the expression of Ngn3, OPN and Thbs4. This likely contributes to embryonic cell differentiation and migration. Human ONECUT2 is 504 amino acids (aa) in length. It contains two DNA binding regions, one classified as a CUT domain (aa 331‑410) and another as a homeobox domain (aa 427-481). There is one potential alternative start site at Met20. Over aa 185-326, human ONECUT2 shares 98% aa identity with mouse ONECUT2.
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
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Cited:
Label
Antibody Source
Product Specifications
Immunogen
Gln185-Thr326
Accession # O95948
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human ONECUT2/OC‑2 Antibody
ONECUT2/OC-2 in HepG2 Human Cell Line.
ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ONECUT2/OC‑2 in HeLa Human Cell Line.
ONECUT2/OC‑2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ONECUT2/OC‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Applications for Human ONECUT2/OC‑2 Antibody
Immunocytochemistry
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using AF6294 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ONECUT2/OC-2
Long Name
Alternate Names
Gene Symbol
UniProt
Additional ONECUT2/OC-2 Products
Product Documents for Human ONECUT2/OC‑2 Antibody
Certificate of Analysis
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Product Specific Notices for Human ONECUT2/OC‑2 Antibody
For research use only
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Citations for Human ONECUT2/OC‑2 Antibody
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: OVCAR-3 human ovarian carcinoma cell lineSpecies: HumanVerified Customer | Posted 09/10/2021
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
