Human ONECUT2/OC-2 Antibody

Catalog # Availability Size / Price Qty
AF6294
AF6294-SP
Human ONECUT2/OC-2 Antibody in Immunocytochemistry
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Product Details
Citations (6)
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Human ONECUT2/OC-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ONECUT2/OC-2 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ONECUT1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ONECUT2/OC-2
Gln185-Thr326
Accession # O95948
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry ONECUT2/OC-2 in HepG2 Human Cell Line. View Larger

ONECUT2/OC-2 in HepG2 Human Cell Line. ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ONECUT2/OC-2

ONECUT2 (one "cut" domain family member 2; also OC-2 and HNF6 beta ) is a monomeric 54 kDa (predicted) member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic neural crest plus hepatic, pancreatic, and intestinal tissue. In the adult, it is found in hepatocytes and small intestine epithelial cells. ONECUT2 is a transcriptional regulator that is known to impact the expression of Ngn3, OPN and Thbs4. This likely contributes to embryonic cell differentiation and migration. Human ONECUT2 is 504 amino acids (aa) in length. It contains two DNA binding regions, one classified as a CUT domain (aa 331‑410) and another as a homeobox domain (aa 427-481). There is one potential alternative start site at Met20. Over aa 185-326, human ONECUT2 shares 98% aa identity with mouse ONECUT2.

Long Name
One Cut Homeobox 2
Entrez Gene IDs
9480 (Human); 225631 (Mouse)
Alternate Names
one cut domain family member 2; HNF-6B; OC2; OC-2; one cut homeobox 2; ONECUT2

Product Datasheets

Citations for Human ONECUT2/OC-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits
    Authors: M Toch, A Harris, O Schakman, E Kondratska, JL Boulland, N Dauguet, S Debrulle, C Baudouin, M Hidalgo-Fi, A Gow, JC Glover, F Tissir, F Clotman
    Sci Rep, 2020;10(1):996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice
    Authors: L Janeckova, K Kostovciko, J Svec, M Stastna, H Strnad, M Kolar, T Hudcovic, J Stancikova, J Tureckova, N Baloghova, E Sloncova, K Galuskova, H Tlaskalova, V Korinek
    Int J Mol Sci, 2019;20(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Rb1/Rbl1/Vhl loss induces mouse subretinal angiomatous proliferation and hemangioblastoma
    Authors: R Wei, X Ren, H Kong, Z Lv, Y Chen, Y Tang, Y Wang, L Xiao, S Hacibekiro, C Liang, A Nagy, R Bremner, D Chen
    JCI Insight, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Rb is required for retinal angiogenesis and lamination
    Authors: Y Zhou, R Wei, L Zhang, Y Chen, S Lu, C Liang, Y Wang, L Xiao, J Zhang, R Bremner, D Chen
    Cell Death Dis, 2018;9(3):370.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development
    Authors: KU Kabayiza, G Masgutova, A Harris, V Rucchin, B Jacob, F Clotman
    Front Mol Neurosci, 2017;10(0):157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells
    Authors: Silvia Velasco
    Cell Stem Cell, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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