Human ONECUT2/OC-2 Antibody
Human ONECUT2/OC-2 Antibody Summary
Gln185-Thr326
Accession # O95948
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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ONECUT2/OC-2 in HepG2 Human Cell Line. ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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ONECUT2/OC‑2 in HeLa Human Cell Line. ONECUT2/OC‑2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ONECUT2/OC‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ONECUT2/OC-2
ONECUT2 (one "cut" domain family member 2; also OC-2 and HNF6 beta ) is a monomeric 54 kDa (predicted) member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic neural crest plus hepatic, pancreatic, and intestinal tissue. In the adult, it is found in hepatocytes and small intestine epithelial cells. ONECUT2 is a transcriptional regulator that is known to impact the expression of Ngn3, OPN and Thbs4. This likely contributes to embryonic cell differentiation and migration. Human ONECUT2 is 504 amino acids (aa) in length. It contains two DNA binding regions, one classified as a CUT domain (aa 331‑410) and another as a homeobox domain (aa 427-481). There is one potential alternative start site at Met20. Over aa 185-326, human ONECUT2 shares 98% aa identity with mouse ONECUT2.
Product Datasheets
Citations for Human ONECUT2/OC-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Single-Cell Transcriptomic Comparison of Human Fetal Retina, hPSC-Derived Retinal Organoids, and Long-Term Retinal Cultures
Authors: A Sridhar, A Hoshino, CR Finkbeiner, A Chitsazan, L Dai, AK Haugan, KM Eschenbach, DL Jackson, C Trapnell, O Bermingham, I Glass, TA Reh
Cell Rep, 2020;30(5):1644-1659.e4.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits
Authors: M Toch, A Harris, O Schakman, E Kondratska, JL Boulland, N Dauguet, S Debrulle, C Baudouin, M Hidalgo-Fi, A Gow, JC Glover, F Tissir, F Clotman
Sci Rep, 2020;10(1):996.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice
Authors: L Janeckova, K Kostovciko, J Svec, M Stastna, H Strnad, M Kolar, T Hudcovic, J Stancikova, J Tureckova, N Baloghova, E Sloncova, K Galuskova, H Tlaskalova, V Korinek
Int J Mol Sci, 2019;20(7):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Rb1/Rbl1/Vhl loss induces mouse subretinal angiomatous proliferation and hemangioblastoma
Authors: R Wei, X Ren, H Kong, Z Lv, Y Chen, Y Tang, Y Wang, L Xiao, S Hacibekiro, C Liang, A Nagy, R Bremner, D Chen
JCI Insight, 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Rb is required for retinal angiogenesis and lamination
Authors: Y Zhou, R Wei, L Zhang, Y Chen, S Lu, C Liang, Y Wang, L Xiao, J Zhang, R Bremner, D Chen
Cell Death Dis, 2018;9(3):370.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development
Authors: KU Kabayiza, G Masgutova, A Harris, V Rucchin, B Jacob, F Clotman
Front Mol Neurosci, 2017;10(0):157.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells
Authors: Silvia Velasco
Cell Stem Cell, 2016;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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