Human ORM2 Antibody (Clone 959445)

Catalog # Availability Size / Price Qty
MAB9306-100
MAB9306-SP
ORM2 in BG01V Human Embryonic Stem Cells.
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Human ORM2 Antibody (Clone 959445) Summary

Species Reactivity
Human
Specificity
Detects human ORM-2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 959445
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ORM-2
Met1-Glu201
Accession # P19652
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ORM2 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

ORM2 in BG01V Human Embryonic Stem Cells. ORM2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry ORM2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

ORM2 in HepG2 Human Cell Line. ORM2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of ORM2 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ORM2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ORM2

Orosomucoid 2 (ORM2) belongs to the family of lipocalin cytosolic fatty-acid binding proteins.  ORM2 is a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. ORM2 may play a role in immunosuppression.

Long Name
Orosomucoid 2
Entrez Gene IDs
5005 (Human)
Alternate Names
AGP 2; AGP2; AGP2alpha-1-acid glycoprotein 2; AGP-B; AGP-B'; alpha-1-acid glycoprotein, type 2; OMD 2; ORM2; orosomucoid 2; orosomucoid-2

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Human ORM2 Antibody (Clone 959445)
By Anonymous on 02/05/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HepG2 Human Cell Line Species: Human