Human ORM2 Antibody (Clone 959445) MAB9306-100: R&D Systems

Human ORM2 Antibody (Clone 959445)

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ORM-2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 959445
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human ORM-2
    Met1-Glu201
    Accession # P19652
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
ORM2 in BG01V Human Embryonic Stem Cells. ORM2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 10 µg/mL for
3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of ORM2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Immunocytochemistry
ORM2 in HepG2 Human Cell Line. ORM2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ORM2
Orosomucoid 2 (ORM2) belongs to the family of lipocalin cytosolic fatty-acid binding proteins.  ORM2 is a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. ORM2 may play a role in immunosuppression.
  • Long Name:
    Orosomucoid 2
  • Entrez Gene IDs:
    5005 (Human)
  • Alternate Names:
    AGP 2; AGP2; AGP2alpha-1-acid glycoprotein 2; AGP-B; AGP-B'; alpha-1-acid glycoprotein, type 2; OMD 2; ORM2; orosomucoid 2; orosomucoid-2
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