Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 159403
View all OX40 Ligand/TNFSF4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human OX40 Ligand
Gln51-Leu183
Accession # P23510

Specificity

Detects human OX40 Ligand in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhCD27 Ligand, recombinant mouse (rm) CD27 Ligand, rhCD30 Ligand, rmCD30 Ligand, rhCD40 Ligand, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, recombinant cotton rat TNF-alpha, rhTNF-alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, rhTRAIL, rhTRANCE, rmTRANCE, or rhVEGF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody

Detection of OX40 Ligand/TNFSF4 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of OX40 Ligand/TNFSF4 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human GM-CSF (Catalog # 215-GM) and LPS were stained with Mouse Anti-Human OX40 Ligand/TNFSF4 APC-conjugated Monoclonal Antibody (Catalog # FAB10541A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human GM-CSF (Catalog # 215-GM) and LPS

Reviewed Applications

Read 1 review rated 4 using FAB10541A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein designated TNFSF4 within the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Like other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the group of co-stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4-1BB Ligand. OX40 appears as a late activation-induced T cell surface antigen, and its major function of OX40-OX40L interaction may be to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

References

  1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.
  2. Baum, P.R. et al. (1994) EMBO J. 13:3992.
  3. Al-Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
  4. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.

Alternate Names

CD252, gp34, TNFSF4

Entrez Gene IDs

7292 (Human); 22164 (Mouse); 89814 (Rat)

Gene Symbol

TNFSF4

UniProt

P23510

Additional OX40 Ligand/TNFSF4 Products

Product Documents for Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody

For research use only

Citations for Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody

Customer Reviews for Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody (1)

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  • Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. <br />Buffer: 2% human serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody FAB10541A

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