Human OX40 Ligand/TNFSF4 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
  • Specificity
    Detects human OX40 Ligand in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhCD27 Ligand, recombinant mouse (rm) CD27 Ligand, rhCD30 Ligand, rmCD30 Ligand, rhCD40 Ligand, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, recombinant cotton rat TNF-alpha, rhTNF-alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, rhTRAIL, rhTRANCE, rmTRANCE, or rhVEGF is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 159403
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human OX40 Ligand
    Accession # P23510
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of OX40 Ligand/TNFSF4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human GM‑CSF (Catalog # 215-GM) and LPS for 48 hours were stained with Mouse Anti-Human OX40 Ligand/TNFSF4 PE‑conjugated Monoclonal Antibody (Catalog # FAB10541P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein designated TNFSF4 within the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Like other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the group of co-stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4-1BB Ligand. OX40 appears as a late activation-induced T cell surface antigen, and its major function of OX40-OX40L interaction may be to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

  • References:
    1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.
    2. Baum, P.R. et al. (1994) EMBO J. 13:3992.
    3. Al-Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
    4. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
  • Entrez Gene IDs:
    7292 (Human); 22164 (Mouse); 89814 (Rat)
  • Alternate Names:
    CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells.
    Authors: Wang C, Yi T, Qin L, Maldonado R, von Andrian U, Kulkarni S, Tellides G, Pober J
    J Clin Invest, 2013;123(4):1677-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Upregulation of OX40 ligand on monocytes contributes to early virological control in patients with chronic hepatitis C.
    Authors: Zhang J, Wu X, Yang B, Wang Y, Feng G, Jiang T, Zeng Q, Xu X, Li Y, Jin L, Lv S, Zhang Z, Fu J, Wang F
    Eur J Immunol, 2013;43(7):1953-62.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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