Human p27/Kip1 IHC Detection Kit

Discontinued Product

CTS2256 has been discontinued.
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Human p27/Kip1 IHC Detection Kit Summary

p27/Kip 1 IHC Detection Kit 

The cyclin-dependent kinase (cdk) inhibitor p27, also known as Kip1, is a tumor suppressor protein. Loss of p27 function has been linked to genetic instability and the progression of many types of cancer. R&D Systems’ Human p27 Immunohistochemistry Detection Kit (Catalog # CTS2256) includes all reagents required for excellent immunoreactivity and localization of the human p27 antigen in formalin-fixed tissues.

The antibody included detects p27 in human and mouse tissues. Specificity was confirmed by comparing staining on tissue sections of normal versus p27 knockout mice and by Western blot on human prostate tissue lysates. Heat-Induced Epitope Retrieval (HIER) pretreatment is required for the detection of this antigen in formalin-fixed, paraffin-embedded (FFPE) sections of human and mouse normal and cancer tissues.

Features

  • Optimized staining reagents
  • Highly consistent
  • Suitable for high-throughput clinical studies

Kit Contents

  • Antigen Retrieval Reagents
  • Blocking Reagents
  • Biotinylated Primary Antibody
  • HRP/DAB Visualization Reagents

 

p27 in Human Prostate Cancer. p27 in Human Prostate Cancer. p27 was detected in paraffin-embedded human prostate cancer sections using R&D Systems’ Human p27 Immunohistochemistry Detection Kit (Catalog # CTS2256). Tissue was stained using anti-mouse HRP-DAB (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

 

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27/Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4-imidazolecarboxamide riboside) or glucose withdrawal.

Specifications

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
Immunohistochemistry
Species
Human

Product Datasheets

Background: p27/Kip1

Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Entrez Gene IDs
1027 (Human); 12576 (Mouse); 83571 (Rat)
Alternate Names
CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1

Reagents Provided

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